骨髓间充质干细胞细胞膜片复合富血小板血浆促进种植体周围骨组织再生的研究

Bone regeneration around dental implant with bone marrow mesenchymal stem cells sheet and platelet rich plasma

目的:研究骨髓间充质干细胞(BMSCs)细胞膜片聚集体与富血小板血浆(PRP)复合凝胶在种植体周围骨组织再生的能力。方法:全骨髓贴壁法培养SD大鼠BMSCs,诱导为细胞膜片并切割为1 mm2大的碎片(BMSCs);采集SD大鼠新鲜动脉血制备PRP;将BMSCs膜片与PRP复合后注入纯Ti种植体与β-TCP材料间隙中(BMSCs+PRP),分别植入裸鼠皮下(n=6);8周后取材观察成骨情况。结果:Micro CT及硬组织切片均显示BMSCs+PRP组在种植体周围有更多的新生骨形成,而单纯BMSCs膜片及单纯PRP组几乎无新生骨形成。结论:BMSCs膜片与PRP联合使用可以促进β-TCP种植体周围骨组织的再生。

Objective： To study the bone regeneration capacity of the BMSCs cell sheets combined with PRP used with dental im- plant. Methods ： BMSCs were isolated from young SD rats and induced to fol^n cell sheets （BMSCs） ; PRP were prepared from the fresh blood of rats; PRP gel with BMSCs fragments（ BMSCs ＋ PRP） were injected into the space of dental implant of Ti and [β-TCP. BMSCs, PRP and BMSCs ＋ PRP with the implant were respectively transplanted into nude mice（ n = 6）. 8 weeks after transplantation bone regeneration were examined by Micro CT and hard tissue slicing. Results ： The group of BMSCs ＋ PRP showed more new bone formation around implants with blood vessel than the group of BMSCs and PRP. Conclusion ： BMSCs sheets with PRP can improve the bone regeneration around dental implants.