IL-1β激活p38 MAPK通路促进人颞下颌关节滑液间充质干细胞分泌IL-6和IL-8

p38 MAPK pathway promotes IL-6 and IL-8 secretion of synovial fluid mesenchymal stem cells from temporomandibular joint inflamed by IL-1β

目的:探讨p38 MAPK信号通路在IL-1β介导下人颞下颌关节滑液间充质干细胞(synovial fluid derived mesenchymal stem cells,SFMSCs)炎性因子分泌中的作用。方法:体外培养SFMSCs,分为对照组和IL-1β(10 ng/ml 2 h)处理组,Western blot检测p38 MAPK蛋白表达,RT-PCR和炎症细胞因子CBA检测对照组、IL-1β处理组和p38 MAPK抑制剂组(SB-203580 10μmol/L、1 h后加入IL-1β10 ng/ml 2 h)h SFMSCs及培养基中IL-6和IL-8的表达和分泌。结果:IL-1β处理组SFMSCs p-p38MAPK蛋白表达、IL-6和IL-8的基因表达及分泌量均明显增加,而p38 MAPK抑制剂组则均明显减少。结论:p38 MAPK信号通路参与炎性微环境下人SFMSCs的炎性分泌。

Objective： To investigate the role of p38 MAPK pathway in IL-1β induced inflammatory secretory properties mesenchymal stem cells（SFMSCs） from human synovial fluid of temporomandibular joint. Methods： In vitro cultured bSFMSCs were divided into control group and IL-1β treated group（ 10 ng/ml IL-1β for 2 h） , the activation of p38 MAPK was examined with western blot. Then, the gene expression and the secretion of IL-6 and IL-8 in control group, IL-1β treated group and p38 MAPK inhibitor pre-treated group （pretreatment with SB-203580 10μmo]/L prior to the addition of 10 ng/ml IL-1β）were examined by RT-PER and cytometrie bead array（CBA）. Results： It was observed that the protein level of p-p38 MAPK, the secretion of IL-8 and IL-6 were increased in IL-1β treated group, and suppressed in p38 MAPK inhibitor pre-treated group. Conclusion： p38 MAPK pathway of SFMSCs might play a role in IL-6 and IL-8 secretion by SFMSCs in inflammatory environment.