蝉花宝牌蝉花片免疫功能评价

Evaluating the immunity function of Chanhuabao tablet

将蝉花宝牌蝉花片(商品名)设低、中、高3个剂量组,分别相当人体推荐量的5、10、30倍,同时设阴性对照组(1%羧甲基纤维素钠),连续1个月经口给予小鼠不同剂量的样品,进行迟发型超敏反应(DTH)、腹腔巨噬细胞吞噬鸡红细胞、抗体生成细胞检测、自然杀伤(NK)细胞杀伤活性和脾淋巴细胞转化等实验,以评估其免疫功能。结果表明,与阴性对照组比较,受试3个剂量组足跖肿胀度极显著增加(P<0.01);中、高剂量组溶血空斑数和血清溶血素抗体积数值均极显著增加(P<0.01),腹腔巨噬细胞吞噬鸡红细胞的吞噬率、吞噬指数显著增加(P<0.05,P<0.01);高剂量组NK细胞活性显著升高(P<0.05)。蝉花宝牌蝉花片(商品名)具有显著的免疫调节功能。

Mice were divided into one negative group（given 1% sodium carboxymethyl cellulose） and three treatment groups at different doses, i.e., low-dose, middle-dose and high-dose groups. Mice were fed with Chanhuabao tablets by five, ten and thirty times of the dose recommended to human beings for 4 weeks, respectively. The abilities of natural killer（NK） cells were tested. The delayed type hypersensitivity（DTH） test and splenic lymphocyte proliferation test were carried out thereafter. The results showed that compared to the negative group, the three treatment groups showed significant swelling of mice（P0.01）. The level of serum hemolysin（P0.01）, hemolytic plaque number（P0.01） as well as the phagocytosis rate and phagocytic index（P0.01） were significantly improved in the middle-and high-dose groups. The killing activity of NK cell was significantly increased in the high-dose group（P0.05）. In conclusion, Chanhuabao tablet could significantly improve the immunological function of mice.