糖尿病足感染的病原菌分布与耐药性分析

Bacterial distribution and resistance in diabetic foot infection

目的了解糖尿病足感染细菌的分布及耐药状况。方法收集2010年至2017年门诊和住院患者的糖尿病足感染标本,常规方法进行细菌培养和鉴定,按纸片法、微量稀释法或E-test法测定细菌药物敏感性。结果共分离出非重复细菌304株,排在前4位的依次是金黄色葡萄球菌、铜绿假单胞菌、无乳链球菌和奇异变形杆菌,分别占29.3%,8.6%,5.9%,5.6%。未检出万古霉素耐药的革兰氏阳性细菌,未检出碳青霉烯类耐药的革兰氏阴性细菌。结论糖尿病足感染的主要致病菌仍以金黄色葡萄球菌为主。

Objective To investigate the bacterial distribution and resistance to antibiotic in diabetic foot infection （DFI）. Methods All the stains of outpatients and inpatients were isolated from infectious tissue in 2010- 2017. The stains were cultured and identified by conventional method, and their susceptibility were tested by disk diffusion, microbroth dilution or E - test according to Clinical and Laboratory Standard Institute （CLSI, 2015） manual Results A total of 304 strains were collected. The four main species were Staphylococcus aureus, Pseudomonas aeruginosa, Streptococcus agalaetiae, and Proteus mirabilis, accounting for 29.3% , 8.6% , 5.9% and 5.6% respectively. There was no resistance to vancomycin for gram -positive bacteria, and no resistance to carbapenem for gram - nagetive bacteria. Conclusion Staphylococcus aureus was predominant organism in DFI.