四逆散干预创伤后应激障碍及睡眠障碍大鼠海马的超微结构研究

Study on intervene function of Sinisan with the ultra structure of hippo-campus in rats with sleep disorder induced by post traumatic stress disorder

目的研究四逆散对创伤后应激障碍导致大鼠睡眠障碍的干预作用。方法按照体重将大鼠随机分为5组,每组10只:空白对照组、模型组、阴性对照组、阳性对照组、实验组。除了空白对照组以外,其他4组动物均制备模型。空白对照组和模型组不给药,阴性对照组灌胃等量0.9% NaCl,阳性对照组灌胃盐酸帕罗西汀溶液0.42 mg·m L^(-1),实验组灌胃四逆散水煎液(含生药0.24g·mL^(-1))。于应激造模前1 h,每组大鼠灌胃给药10 mL·kg^(-1),每天灌服1次,共计7 d。用透射电镜观察比较各组大鼠给药前后的海马CA1区、CA3区超微结构改变。结果空白对照组的海马CA1、CA3区神经元突触结构清晰,突触间隙明显,突触小泡可见。与空白对照组比较,模型组的海马CA1、CA3区神经元突触结构明显改变,突触小泡减少,突触结构不清晰。与模型组比较,阴性对照组变化与其相似,提示0.9%NaCl灌胃对大鼠没有产生明显的影响。与阴性对照组比较,阳性对照组和实验组的海马CA1神经元突触结构明显恢复,且2组变化相似。结论用4万倍透射电镜观察的创伤后应激障碍所致睡眠障碍大鼠的海马CA1、CA3区超微结构特征性强。

Objective To study on intervene function of Sinisan with the ultra structure of hippo - campus in rats with sleep disorder induced by post traumatic stress disorder. Methods Fifty SD rats were divided equally into five groups： blank control group, model group, negative control group, positive control group and experimental group. The control blank group did not copy the model, do not receive drugs, the normal feeding. Model group, repetitive post -traumatic stress disorder model was induced by current stimulation, but not receive drugs. Negative control group, equal volume of 0. 9% NaCl. Positive control group （ paroxetine hydrochloride 0. 42 mg·mL-l ） and experimental group （ Sinisan, containing crude drug 0. 24 g · mL-1 ）. It given to drugs 1 h before the model establishment. The rats were administered with 10 mL·kg-1, once a day, for a total of 7 d. The changes of ultra structure of CA1 and CA3 area in hippo - campus with rats were observed by transmission electronmicroscope. Results In the blank control group, the hippocampal neurons were clearly defined, and the synaptie space was obvious in the CA1 and CA3 area. Compared with the blank control group, in the model group, the synaptic structure of hippocampal neurons was significantly changed, the synaptic vesicles were reduced and the synaptic structure was not clear in the CA1 and CA3 area. Compared with the model group, the negative control group was same too, which indicated that there was no significant stress effect on the rats after intragastric administration of saline. Compared with the negative control group, positive control group and experimental group hippocampal neurons synapse structure was restored, and the two groups were similar in the CAland CA3 area. Conclusion Transmission electronmicroscope was used to study the ultra structure of CA1 and CA3 in rats hippo - campus with characteristic difference.