棘孢木霉液体发酵条件优化

Optimization of liquid fermentation conditions of Trichoderma asperellum

为研究棘孢木霉液体发酵最优条件,以菌丝体干重和孢子数为指标,通过单因素正交实验对棘孢木霉液体发酵培养基进行优化,并测定棘孢木霉的基本成分。实验结果表明最佳发酵条件为:黄豆粉0.25%,KH_2PO_4 0.3%,MgSO_4 0.15%,接种量8×10~6个/mL,装瓶量100 mL/250 mL,蛋白胨1.35%,葡萄糖3.5%,转速160 r/min,温度25℃,自然光照,pH7。此发酵条件可使棘孢木霉干重量达到19.453 g/L,孢子数达到4.4812×10~9个/mL。本研究为棘孢木霉的工业化生产降低成本,缩短发酵周期打下理论基础。

To study the optimum conditions for the fermentation of Trichoderma asperellum,the effects of dry weight and spores number on myeelia were optimized by single factor orthogonal experiment.Experimental resuh showed that the best medium was soy flour medium and the optimum conditions were as follows：0.25% of Soy flour,0.3% of KH2PO4 ,0.15% of MgSO4 ,8 ×106/mL of inoculationamount, 100 mL/250 mL of bottling volume, 1.35 % of Peptone, 3.5 % of glucose, 160 r/min of rotating speed,25℃ , natural light, and primary pH7.The fermentation conditions can make the dry weight of Trichoderma viride reach 19.453 g/L,the number of spores reached 4.4812 ×109/mL. For the mass production of Trichoderma asperellum reduced the cost, shorten the fermentation cycle,laying the theoretical basis for the industrial production of Trichoderma aspereUum.