竹子超声波萃取液特性及其漆酶催化研究

Bamboo ultrasonic extractions and its laccase catalysis

竹子属于木质纤维素的一种，其主要成分为纤维素、半纤维素和木质素。超声波是一种频率在20—50kHz范围内的声波，超声波提取就是利用超声波产生的空化、振动、粉碎和搅拌的综合效应对天然产物中的有效成分进行萃取。利用超声波对竹子的萃取、漆酶催化超声波萃取，构建竹子-超声波处理体系和竹子-漆酶催化氧化体系，研究超声波对竹子的萃取效果和漆酶对竹子超声波萃取液的催化氧化效果。通过分析扫描电镜、傅里叶红外变换光谱和紫外分光测试结果表明：超声波作为一种环保温和的处理方式可以将竹子中部分木素酚类分子提取出来；漆酶可以催化氧化超声波萃取液，生成新的物质，且对环境无污染。

Bamboo is a kind of lignocellulose material, and its main components are cellulose, hemicellulose and lignin. Ultrasonic is a sound wave with frequency in the range of 20- 50 kHz. Ultrasonic extraction takes the use of ultrasonic cavitation, vibration, crushing and the combined effect of stirring on the extraction of active ingredients from natural resources. The ultrasonic extraction of bamboo with laccase catalysisis focused on. The bamboo-ultrasonic system and bamboo ultrasonic-laccase catalyzing system are constructed. The ultrasonic extraction on bamboo and the laccase catalysis effect are studied. The results with scanning electron microscopy, Fourier transform infrared spectroscopy and UV spectroscopy indicate that ultrasonic treating is an environmentally friendly pretreatment method to extract part of bamboo lignin phenols from bamboo, and the use of laccase can catalyze and have oxidation on the bamboo ultrasonic extraction to produce new substances without pollution.