miR-26调控BID蛋白对结肠癌细胞增殖和侵袭的影响

Effects of miR-26 on the proliferation and invasion of colon cancer cells by BID protein

目的探讨miR-26对BID蛋白的调控作用及在结肠癌细胞中对其增殖和侵袭能力的作用。方法 qPCR检测结肠癌组织和结肠癌细胞中miR-26的表达情况;探讨miR-26和结肠癌临床病理参数之间的关系;免疫荧光检测miR-26-inhibitor在结肠癌细胞中的转染效率;双荧光素酶实验检测miR-26和BID的关系;MTT实验检测沉默miR-26对结肠癌细胞增殖能力的影响;Transwell侵袭实验检测沉默miR-26对结肠癌细胞侵袭能力的影响。结果 miR-26在结肠癌组织中表达水平较高;随着结肠癌的病理分期升高,miR-26的表达相应升高;沉默miR-26可以有效上调BID的表达;沉默miR-26可以抑制结肠癌细胞的增殖和侵袭能力。结论 miR-26在结肠癌中表达上调,同时miR-26可以调控BID的表达影响结肠癌细胞增殖和侵袭能力。

Objective To investigate the effect of miR-26 on the regulation of BID protein and its effect on proliferation and invasion of colon cancer cells. Methods The expressions of miR-26 in colon cancer tissues and colon cancer cells were detected by q PCR. The relationship between miR-26 and clinicopathological staging of colon cancer was examined. Immunofluorescence was used to detect the transfected efficiency of miR-26-inhibitor in colon cancer cells.MTT assay was used to detect the effect of miR-26 on the proliferation of colon cancer cells. Transwell invasion assay was used to detect the effect of miR-26 on the invasion of colon cancer cells. Results The expression of miR-26 in colon cancer tissue was higher than that in normal tissue. The expression of miR-26 was significantly increased with the pathological stage of colon cancer. Silencing miR-26 could improve the expression of BID. Silencing miR-26 could inhibit proliferation and invasive ability of colon cancer cells. Conclusion miR-26 is up-regulated in colon cancer,and miR-26 can regulate the expression of BID and affect the proliferation and invasion of colon cancer cells.