带状疱疹后神经痛模型小鼠脊髓的自噬变化

Changes of autophagy levels in spinal cord of mice with postherpetic neuralgia

目的:观察带状疱疹后神经痛(postherpetic neuralgia,PHN)模型小鼠脊髓自噬程度的变化。方法:36只昆明小鼠随机分为生理盐水组(NS)、溶剂组(Solvent)与腹腔注射树脂毒素组(RTX),每组12只。RTX组0.2μg/g的剂量构建PHN模型,Solvent组、NS组分别注射等体积的溶剂和生理盐水。每天定时用von-frey纤维丝检测机械缩足阈值(PWMT),热板法检测缩足时间阈值(HPPT)至疼痛稳定。然后分别处死各组小鼠,取脊髓L4—L6节段使用实时荧光定量PCR、免疫组织化学染色及western Blot等方法检测LC3、beclin1的变化情况。结果:使用0.2μg/g的RTX后PHN模型PWMT显著降低、HPPT显著增加(P<0.05),同时自噬相关蛋白LC3、beclin1的mRNA和蛋白相对表达量显著性增加(P<0.05)。结论:0.2μg/g的RTX可以复制PHN小鼠模型;PHN小鼠模型脊髓自噬程度增加。

Objective： To observe the changes of autophagy in the spinal cord of postherpetic neuralgia （PHN） mice. Method： Thirty-six Kunming mice were randomly divided into group normal saline, group solvent and group PHN，12 mice in each. They were treated with equal volume intraperitoneal injection of normal saline, solvent and 0.2μg/g resiniferotoxin solvent separately。Von-frey fiber was used to detect the paw mechanical thresholds. The hot plate method was used to detect the shrinkage time threshold at the same time every day for every mice. The expression of LC3 and beclin1 was detected by real-time fluorescence quantitative PCR, immunohistochemistry and Western-Blot in the spinal cord segment of L4—L6 after day 7. Result： The PWMT value decreased and HPPT increased significantly （P＜0.05） in group PHN after intraperitoneal injection of 0.2μg/g resiniferotoxin, while the level of mRNA and protein, the relative expression of LC3, beclin1 significantly increased （P＜0.05）. Conclusion： Intraperitoneal injection of 0.2μg/g RTX can replicate PHN mice model. Levels of autophagy Increased in PHN mice.