黄瓜叶面喷施硅肥对果实糖分和维生素C积累及相关酶的影响

Effects of Foliar Application of Silicon on Accumulation of Sugar and Vitamin C and Related Enzymes in Cucumber Fruits

研究了黄瓜叶面喷施硅肥对其生长、产量、果实糖分、维生素C及相关酶活性和基因表达量的影响。结果表明:叶面喷施硅肥显著促进了黄瓜生长,增加了单株产量,提高了果实中碱性转化酶(NI)、蔗糖磷酸合成酶(SPS)、蔗糖合成酶(SS)及半乳糖内脂脱氢酶(GaILDH)活性,提高了果实果糖、葡萄糖和维生素C含量。其中,1.420 g·L^(-1)单硅酸处理效应最显著,其果糖、葡萄糖和维生素C含量分别较对照高13.79%、18.08%和75.11%。实时荧光定量PCR分析表明,叶面喷施单硅酸的黄瓜果实碱性转化酶–3(NI-3)、蔗糖磷酸合成酶(SPS)和半乳糖内脂脱氢酶(GaILDH)基因表达量显著提高。

The effects of foliar application of silicon on growth,yield,accumulation of sugar and vitamin C in fruit and activities and gene expression of the related enzymes of cucumber were studied. The results showed that foliar application of silicon significantly promoted the growth and yield of cucumber plants,and increased activities of neutral invertase（NI）,sucrose phosphate synthase（SPS）,sucrose synthase（SS）and galactose dehydrogenase（GaILDH）and contents of fructose,glucose and vitamin C in fruits of cucumber. Among all treatments,foliar application of 1.420 g · L-1 monosilicic acid showed the most significant effect. Compared to the control,contents of fructose,glucose and vitamin C of the fruits increased by 13.79%,18.08% and 75.11%,respectively. Real-time quantitative PCR results indicated that the genes expression of neutral invertase-3（NI-3）,sucrose phosphate synthase（SPS）and galactose dehydrogenase（GaILDH）were significantly increased in fruits under 1.420 g · L^（-1） monosilicic acid treatment.