黄瓜蜡质合成调控基因CsWIN1的克隆与功能初步分析

Cloning and Functional Analysis of CsWIN1, a Transcription Factor Regulated the Wax Synthesis in Cucumber

从黄瓜中同源克隆了拟南芥中具有调控蜡质合成与代谢功能的基因WIN1/SHINE1,命名为CsWIN1。荧光定量PCR结果表明:CsWIN1在黄瓜植株的叶片和幼果中高表达;通过在拟南芥中过表达CsWIN1,发现其与已报道的WIN1/SHINE1过表达株系表型相似。通过对CsWIN1转基因株系的基因表达分析发现,蜡质合成相关基因的表达受到了调控。根据以上研究结果,推测CsWIN1与拟南芥WIN1/SHINE1在表皮蜡质合成调控上的功能是保守的,通过调控下游蜡质合成相关基因的表达从而影响蜡质的合成与代谢。

In Arabidopsis,WIN1/SHINE1 gene has the function of regulating wax synthesis and metabolism. In this study,a homologous gene of WIN1/SHINE1 was cloned from cucumber and named CsWIN1. The results of q RT-PCR showed that CsWIN1 expressed at high levels in the leaves and young fruits of cucumber. Subcellular localization analysis suggested that the CsWIN1 protein is located in the nucleus. The CsWIN1 overexpression in the transgenic plants of Arabidopsis thaliana caused a similar phenotype to WIN1/SHINE1 overexpressing lines of Arabidopsis. The expression analysis of CsWIN1 transgenic lines indicated that the expressions of the genes related to wax synthesis have been changed significantly. These results suggested that the function of CsWIN1 is conserved in the regulation of epidermal wax synthesis through regulating the expression of downstream genes related to wax synthesis to affect wax biosynthesis and metabolism.