磁场对毛蚶肝胰腺抗氧化酶活性的影响

Effect of Magnetic Field on Antioxidant Enzyme Activities of Hepatopancreas in Scapharca subcrenata

为了研究磁场对毛蚶肝胰腺抗氧化酶活性的影响,本研究在实验室条件下分别检测了在3-10 mt及40-90 mt磁场强度下培养8 d、15 d的毛蚶（Scapharca subcrenata）肝胰腺中的过氧化物酶（POD）活性、超氧化物歧化酶（SOD）活性、超氧阴离子自由基（O2^-）的产生速率和丙二醛（MDA）含量。研究发现,在外加磁场的作用下,毛蚶肝胰腺中的O2^-、SOD、POD以及MDA等4项指标均受到磁场的影响;在施加3-10 mt及40-90 mt强度的磁场后,磁场对毛蚶产生了氧化胁迫,且40-90 mt组的应激反应快于3-10 mt组。本实验研究认为,O2^-生成速率、SOD与POD活性可以作为潜在的海洋贝类是否受到外在磁场损伤的监测指标。同时用以提示海洋环境中磁场造成的污染程度,为人类的生产建设活动提供参考。

To study the effect of magnetic field on antioxidant enzyme activities of hepatopancreas in Scapharca subcrenata, activities of peroxidase（POD） and superoxide dismutase（SOD）, generation rate of superoxide anion radical（O2^-） and content of malondialdehyde（MDA） in hepatopancreas were detected after Scapharca subcrenata were exposed to the magnetic fields at the intensity of 3-10 mt and 40-90 mt for 8 days and 15 days under laboratory conditions. The results showed that 4 factors of hepatopancreas in Scapharca subcrenata, including O2^-,SOD, POD and MDA, were affected by the magnetic field. After the magnetic field at the intensity of 3-10 mt and40-90 mt was applied, oxidative stress on Scapharca subcrenata was observed and the stress reaction in the 40-90 mt group was faster than that in the 3-10 mt group. The results suggested that generation rate of O2^-, activities of SOD and POD could be used as potential monitoring indexes indicating whether marine shellfish were damaged by external magnetic field. They could also indicate the degree of magnetic pollution in the marine environment and provide references for human＇s production and construction activities at the same time.