摘要
查尔酮异构酶（chalcone isomerase,CHI）是花青素生物合成途径中的一个关键酶。本研究根据转录组数据,利用RT-PCR技术在甘薯中克隆了一个新的CHI基因,命名为IbCHIL1。IbCHIL1基因c DNA长857 bp,包含1个621 bp的开放阅读框,编码206个氨基酸。聚类分析表明,IbCHIL1属于Ⅳ型CHI成员,和其他植物中的类似蛋白具有很高的同源性。表达分析显示,IbCHIL1主要在紫肉甘薯中表达,与花青素积累正相关,推测该基因在甘薯花青素生物合成过程中具有重要的生物学功能。
The chalcone isomerase is a key enzyme in anthocyanin biosynthesis pathway. A new chalcone isomerase gene named IbCHIL1 was cloned in Ipomoea batatas based on transcriptome data and RT-PCR technology. The full-length of c DNA was 857 bp, containing 621 bp opening reading frame（ORF）, which encoded 206 amino acids. Phyogenetic analysis indicated that IbCHIL1 belonged to the type Ⅳ CHI group, and showed high identity to similar proteins in other plants. The expression results showed that IbCHIL1 was mainly expressed in purple-fleshed sweet potato, positively related to the accumulation of anthocyanins. The results showed that IbCHIL1 might play an important biological function in anthocyanin biosynthesis of sweet potato.
作者
徐靖
朱家红
韩义胜
白翠云
朱红林
Xu Jing 1, Zhu Jiahong 2, Han Yisheng 1, Bai Cuiyun 1 ,Zhu Honglin 1(1 Institute of Cereal Crops, Hainan Academy of Agricultural Sciences, Key Laboratory of Crop Genetics and Breeding of Hainan Province, Scientific Observation Station for Gene Resources and Germplasm Creation of Hainan, Ministry of Agriculture, Haikou, 571100; 2 Institute of Tropical Bio- sciences and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou, 57110)
出处
《基因组学与应用生物学》
CAS
CSCD
北大核心
2018年第2期845-849,共5页
Genomics and Applied Biology
基金
海南省重点研发计划项目（ZDYF201606）
海南省农业科学院农业科技创新专项（CXZX201613）共同资助
关键词
甘薯
查尔酮异构酶
花青素
基因表达
Sweet potato, Chalcone isomerase, Anthocyanin, Gene expression
