克拉玛依油田工人职业紧张与ADD1基因对高血压患病率的影响

Effects of occupational stress and ADD1 gene on prevalence of hypertension in Karamay oilfield workers

[目的]探讨克拉玛依油田工人职业紧张和ADD1基因对高血压患病率的影响。[方法]采用随机整群抽样方法抽取克拉玛依某油田在岗作业工人1 300人,运用职业紧张量表进行问卷调查(回收有效问卷1 233份,问卷有效率为94.8%),采集其中352名油田工人(高血压病例组和非高血压对照组各176人)的血样,并提取DNA,运用聚合酶链式反应-限制性片段长度多态性技术检测ADD1基因rs17833172和rs4961位点的基因型。[结果]1 233名油田工人的职业任务得分(168.28±34.19)高于国内常模(162.89±27.04)(P<0.05),且高血压患病率随紧张程度增加而上升(χ_(趋势)~2=13.82,P<0.05),高度紧张组的高血压患病率(18.49%)高于低度紧张组(1.54%)和中度紧张组(5.51%)。在352名采集血样检测ADD1基因的油田工人中,rs4961基因型在不同性别、年龄和工龄组间的分布差异有统计学意义(均P<0.05)。多因素logistic回归分析显示,较高的个体应对资源(OR=0.989,95%CI:0.979~0.998)和rs4961位点TT基因型(OR=0.455,95%CI:0.244~0.846)是高血压的保护因素,rs17833172位点GG基因型(OR=3.113,95%CI:1.402~6.916)是高血压的危险因素(P<0.05)。[结论]油田工人高血压是基因与职业紧张综合作用的结果,较高的个体应对资源和ADD1基因rs4961位点TT基因型可能会降低油田工人高血压发病风险,ADD1基因rs17833172位点GG基因型可能会增加油田工人高血压的发病风险。

[Objective] To investigate the effects of occupational stress and ADD1 gene on the prevalence of hypertension in Karamay oilfield workers.[Methods] A total of 1 300 oilfield workers in Karamay were selected by random cluster sampling method and investigated using Occupational Stress Inventory-Revised （the number of valid questionnaire was 1 233, and the valid rate was 94.8%）. Blood samples were collected from 352 oilfield workers （176 hypertension cases and 176 controls） to extract DNA and detect the genotypes of rs17833172 and rs4961 in ADD1 gene by polymerase chain reaction-restrictive fragment length polymorphism method.[Results] There was a higher score of occupational role in Karamay oilfield workers （168.28±34.19） than that of the domestic norm （162.89±27.04） （P 〈 0.05）. The prevalence rate of hypertension increased with higher occupational stress levels （χtrend2=13.82, P 〈 0.05）, and the prevalence rate was higher in the high-stress group （18.49%） than in the low-stress group （1.54%） and the middlestress group （5.51%）. Among the blood samples of 352 oilfield workers, the genotypes of rs4961 varied among groups categorized by gender, age, and working years between the hypertension case group and the control group （Ps 〈 0.05）. The multi-factor logistic regression analysis results showed that higher personal coping resources （OR=0.989, 95%CI：0.979-0.998） and TT genotype of rs4961 （OR=0.455, 95%CI：0.244-0.846） were protective factors for hypertension, while GG genotype of rs17833172 （OR=3.113, 95%CI：1.402-6.916） was a risk factor （P 〈 0.05）.[Conclusion] Oilfield workers＇ hypertension may result from multi-sourced pathogens related to genes or occupational stress. Higher personal coping resources and rs4961 TT genotype of ADD1 gene may associate with a lower risk of hypertension in the oilfield workers, while rs17833172 GG genotype may associate with a higher risk of hypertension.