中药麦冬高效液相指纹图谱研究

Study on HPLC Fingerprint of Ophiopogon Japonicus

目的:建立中药麦冬的高效液相指纹图谱,并同时测定甲基麦冬二氢高异黄酮A、甲基麦冬二氢高异黄酮B的含量。方法:采用高效液相色谱(high performance liquid chromatography,HPLC)化学定量指纹图谱的方法,建立麦冬的HPLC指纹图谱和甲基麦冬二氢高异黄酮A、甲基麦冬二氢高异黄酮B含量的测定方法。色谱条件为Waters symmetry shieldTMRP 18(4.6 mm×250.0.mm,5μm)色谱柱,symmetry shieldTMRP 18预柱,以乙腈-体积分数0.1%磷酸水溶液为流动相进行梯度洗脱,流速:1.0 mL·min^(-1),检测波长:280 nm,柱温:30℃。结果:麦冬HPLC指纹图谱分离度高、重现性好,共标定了25个共有峰,各批次药材的相似度在0.99以上。通过与对照品比对,甲基麦冬二氢高异黄酮A、甲基麦冬二氢高异黄酮B分别为第14号峰及第15号峰,甲基麦冬二氢高异黄酮A、甲基麦冬二氢高异黄酮B总含量为0.042 0~0.065 5 mg·g^(-1)。结论:本研究所建立的麦冬HPLC指纹图谱灵敏度高、专属性强,可作为麦冬原药材质量控制的依据。

Objective：To establish a HPLC fingerprint of Radix ophiopogonis and to determine the content of methyl-ophiopogon japonicus dihydro-isoflavone A and methyl-ophiopogon japonicus dihydro-isoflavone B simultaneously.Methods：HPLC fingerprint of Radix ophiopogon japonicus was established by high performance liquid chromatography（HPLC）.The HPLC fingerprint of Radix ophiopogon japonicus and the content determination of methyl-ophiopogon japonicus dihydro-isoflavone A and methyl-ophiopogon japonicus dihydro-isoflavone B were established.The chromatographic conditions were Waters symmetry shieldTMRP18（4.6 mm×250.0 mm,5 μm） column,symmetry shieldTMRP18 pre-column with acetonitrile-0.1% phosphoric acid Mobile phase gradient elution,flow rate：1.0 mL·min^（-1）,detection wavelength：280 nm,column temperature：30 ℃.Results：The HPLC fingerprint of ophiopogon japonicus had good resolution and good reproducibility.Collected a total of 25 common peaks,and the similarity of each batch was above 0.99.By comparing with the reference substance,methyl-ophiopogon japonicus dihydro-isoflavone A,methyl-ophiopogon japonicus dihydro-isoflavone B were No.14 peak and No.15 peak,and its quantitative analysis.Conclusion：The HPLC fingerprint of Radix ophiopogon japonicus established in this study has high sensitivity and specificity and can be used as the basis for quality control of Radix ophiopogonis.