疲劳安口服液对慢性疲劳综合征大鼠脾脏指数及行为学的影响

Effect of Pi Lao An Oral Liquid on Spleen Index and Behavior of Rats with Chronic Fatigue Syndrome

目的:观察疲劳安口服液对慢性疲劳综合征大鼠脾脏指数及行为学的影响。方法:54只SD大鼠,随机分为正常组,模型组,对照组,疲劳安口服液大剂量组、中剂量组、小剂量组,造模成功后分别给予质量分数9 g·L-1氯化钠注射液、疲劳安口服液不同剂量、人参皂苷汤灌胃治疗。14 d后观察行为学指标;处死大鼠,分离脾脏,计算脾脏指数。结果:与正常组大鼠比较,模型组大鼠脾脏指数显著降低(P<0.01),而疲劳安口服液大剂量组、中剂量组与正常组及人参皂苷组均无显著变化(P>0.05)。模型组大鼠找到目标区所用的时间显著高于正常组(P<0.05),模型组敞箱实验水平及垂直穿格次数、力竭游泳时间、鼠尾悬挂不动时间显著低于正常组(P<0.05);疲劳安口服液大剂量组、中剂量组大鼠找到目标区所用的时间、敞箱实验水平及垂直穿格次数、力竭游泳时间、鼠尾悬挂不动时间与正常组及人参皂苷组比较,差异均无统计学意义(P>0.05)。结论:疲劳安口服液可升高慢性疲劳综合征大鼠的脾脏指数,降低大鼠找到目标区所用的时间,提高大鼠穿格次数、力竭游泳时间。

Objective： To observe the effect of Pi Lao An Oral Solution on spleen index and behavior of rats with chronic fatigue syndrome. Methods： 54 SD rats were randomly divided into for the normal group,model group,control group as well as high,middle,and low dose of Pi Lao An Oral Liquid. After the successful modeling of them,they were given 0. 9% sodium chloride injection,different doses of Pi Lao An Oral Liquid and Ginseng saponins soup gavage respectively for 14 days. The rats were observed in behaviours and then were sacrificed and the spleens were isolated to calculate the spleen index. Results： the spleen index of rats in model group was significantly lower than that of the normal group（P〈0. 01）,while there is no significant difference in that in Pi Lao An Oral Liquid groups and Ginsenoside group（P〈0. 05）; The period of time in which model group rats to find target zone was significantly higher than that of the normal group（P〈0. 05）,while the number of going through the open box test with horizontal and vertical grid,the exhaustive swimming time,rat tail suspension time was significantly lower than that in the normal group（P〈0. 05）. There is no significant difference in the period of time in which model group rats to find target zone,the number of going through the open box test with horizontal and vertical grid,the exhaustive swimming time,rat tail suspension time between all of the Pi Lao An Oral Liquid groups,and Ginsenoside group（P〈0. 05）. Conclusion： Pi Lao An Oral Liquid can increase the spleen index in rats with chronic fatigue syndrome,decrease the time of finding target zone. It can also increase the number of going through the open box test with horizontal and vertical grid,the exhaustive swimming time of model rates.