杜仲叶醇提取物对去卵巢骨质疏松大鼠骨代谢生化指标、骨密度、IL-6及TNF-α的影响

Effects of Eucommia Leaf Alcohol Extract on Bone Metabolism Biochemical Indexes,Bone Mineral Density,IL-6 and TNF-α in Ovariectomized Rats

目的:观察杜仲叶醇提取物对去卵巢骨质疏松大鼠骨代谢生化指标、骨密度、白细胞介素-6(interleukin-6,IL-6)和肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)的影响。方法:90只清洁级SD雌性大鼠随机分为对照组、假手术组、模型组、雌激素组和杜仲叶组各18只。模型组、雌激素组和杜仲叶组大鼠做去卵巢处理,假手术组同样手术但不切除卵巢。术后4周,对照组、假手术组和模型组大鼠给予蒸馏水灌胃;雌激素组大鼠给予戊酸雌二醇灌胃(0.6 mg·kg^(-1));杜仲叶组大鼠给予杜仲叶醇提取物灌胃(6 g·kg^(-1)),1次·d^(-1),连续8周。检测所有大鼠血清钙、磷,尿钙、磷,骨碱性磷酸酶(bone alkaline phosphatase,BALP),骨钙素,雌二醇,IL-6和TNF-α水平,并测定骨密度。结果:与对照组和假手术组比较,模型组大鼠尿Ca/Cr、P/Cr、BALP、骨钙素、IL-6、TNF-α均显著升高,血清雌二醇含量和骨密度显著降低,差异均有统计学意义(P<0.05);与模型组比较,雌激素组和杜仲叶组大鼠尿Ca/Cr、P/Cr、BALP、骨钙素、IL-6、TNF-α均显著降低,血清雌二醇含量和骨密度显著升高,差异均有统计学意义(P<0.05)。结论:杜仲叶醇提取物对去卵巢骨质疏松大鼠具有较好的疗效,其作用机制可能与降低血清IL-6、TNF-α含量,调节骨代谢平衡,提高骨密度有关。

Objective： to observe the effects of Eucommia leaf alcohol extract on biochemical indexes of bone metabolism,bone mineral density,IL-6 and TNF-α in ovariectomized rats. Methods： 90 female SD rats were randomly divided into control group,sham operation group,model group,estrogen group and eucommia leaf group,with 18 rats in each group. The model group,estrogen group and Eucommia leaf group rats were ovariectomized and the sham operation group underwent the same operation but without ovariectomy. 4 weeks after operation,the control group,sham operation group and model group were given distilled water for gavage; the estrogen group was given intragastric administration of estradiol valerate（0. 6 mg·kg^-1）; the eucommia leaf group was given intragastric administration of ethanol extract of Eucommia ulmoides leaves（6 g·kg^-1）,1 time/day for 8 weeks; the content of calcium and phosphorus in serum and urine,bone alkaline phosphatase（BALP） and Osteocalcin（OCN）,estradiol（E2） and interleukin-6（IL-6） and tumor necrosis factor α（TNF-α） of all rats were measured,and then the bone mineral density（BMD） of all rats were measured. Results： Compared with that of the control group and sham operation group,the content of calcium and phosphorus in rat urine,BALP,OCN,IL-6,and TNF-α in the model group significantly increased,while the content of serum E2 and BMD significantly decreased（P 〈0. 05）. Compared with that of the model group,the content of calcium and phosphorus in rat urine,BALP,OCN,IL-6,and TNF-α of the estrogen group and Eucommia leaf group significantly decreased,while the content of serum E2 and BMD significantly increased（P 〈0. 05）. Conclusion： Eucommia leaf alcohol extract has good effects in treatment of ovariectomized rat,its mechanism may be related to the decrease of serum IL-6 and TNF-α,the improvement of the bone density by regulating the bone metabolism balance.