阿尔兹海默病与HLA-A、-B、-DRB1基因遗传多态性相关性的研究

Association of gene polymorphism of HLA-A,-B,-DRB1 with Alzheimer’s disease

目的探讨辽宁地区散发性阿尔茨海默病（AD）与HLA相关位点基因遗传多态性的关联性。方法采用特异性寡核苷酸序列探针分析法（SSO）检测辽宁地区44名散发性阿尔茨海默病患者（实验组）和90名健康老人（对照组）HLA-A、HLA-B和HLA-DRB1位点基因遗传多态性。应用Arlequin3.5软件进行Hardy-Weinberg平衡、单体型频率的分析;应用SPSS17.0软件进行卡方检验,比较2组间等位基因和单体型频率的差异。结果实验组和对照组HLA-A、B、DRB1位点的各个等位基因频率的差异均不具统计学意义（P〉0.05）;HLA-A-B单体型频率最高的为A＊ 30-B＊ 13、A＊ 02-B＊ 46、A＊ 02-B＊ 15,2组间高频单体型频率的差异也不具统计学意义（P〉0.05）。结论辽宁地区散发性阿尔茨海默病与HLA-A、HLA-B和HLA-DRB1位点基因遗传多态性无相关性。

Objective To explore the connections between gene polymorphism of HLA and Alzheimer＇s disease. Methods A total of 44 Liaoning Han patients with Alzheimer＇s disease and 90 unrelated healthy controls were recruited in this study. SSO method was used to detect the genotypes of HLA-A,HLA-B and HLA-DRB1. Hardy-Weinberg equilibrium were evaluated by chi-square tests. Hardy-Weinberg equilibrium and haplotype frequencies were carried out using Arlequin3. 5 software. Differences in allelic and haplotype frequencies were evaluated by chi-square test using SPSS 17. 0 statistical software. Results No significant differences in all alleles of HLA-A,HLA-B and HLA-DRB1 between the two groups（P〈0. 05）. High frequent haplotypes were A＊ 30-B＊ 13,A＊ 02-B＊ 46,A＊ 33-B＊ 58,no significant differences in these haplotypes between the two groups either（P〉0. 05）. Conclusion Gene polymorphisms of HLA-A,HLA-B and HLA-DRB1 are not likely to be associated with risk of AD in Liaoning Han population.