摘要
目的研究急性心肌梗死(AMI)与稳定型心绞痛(SA)病人体内高密度脂蛋白胆固醇(HDL-C)对内皮功能影响的差异。方法采用梯度离心法提取正常志愿者(C组,n=28)、急性心肌梗死(AMI组,n=25)与稳定型心绞痛病人(SA组,n=25)体内的HDL,然后检测各组HDL对脐静脉内皮细胞(HUVEC)内皮一氧化氮合酶(eNOS)及其磷酸化位点S1177的表达、一氧化氮(NO)及氧自由基(O_2·^-)水平的影响。结果与C组比较,SA组HDL-C对HUVEC内eNOS、NO、及O_2·^-的影响无统计学意义(P>0.05);与SA组比较,AMI组HDL明显抑制HUVEC内eNOS及其磷酸化位点S1177的表达、降低NO水平和增加O_2·^-含量(P<0.05)。结论 AMI病人体内的HDL通过抑制eNOS/NO/O_2·途径损害内皮功能。
Objective To study the effect of high density lipoprotein(HDL-C)on endothelial function in patients with acute myocardial infarction(AMI)or stable angina(SA).Methods Gradient centrifugation was used to centrifuge HDL from normal volunteers(control group,n =28),AMI patients(AMI group,n =25)and SA patients(SA group,n =25).The effects of HDL on endothelial nitric oxide synthase(eNOS)and its phosphorylation sites at S1177 expression,levels of nitric oxide(NO)and oxygen free radical(O2·-)in human umbilical vein endothelial cells(HUVEC)were detected.Results There was no significant difference in eNOS expression,NO and O2·-levels in HUVES between SA group and control group(P 〉0.05).Compared with SA group,the expression of eNOS and its phosphorylation sites at S117 were inhibited,NO level decreased,and the O2·-content increased in AMI group(P 〈0.05).Conclusion HDL-C damage endothelial function by inhibiting eNOS/NO/O2·-pathway in AMI patients.
作者
李芸
曹德勇
LI Yun, Cao Deyong(Cardiovascular Specialist Branch, The Affiliated Hospital of Yan' an University, Yan'an 716000, Shaanxi, Chin)
出处
《中西医结合心脑血管病杂志》
2018年第3期315-319,共5页
Chinese Journal of Integrative Medicine on Cardio-Cerebrovascular Disease
基金
国家自然科学基金专项基金项目(No.1341112)