EP4基因沉默对甲状腺乳头状癌K1细胞生长及迁移的影响

Effect of EP4 gene silencing on the growth and migration of papillary thyroid carcinoma K1 cells

目的探讨EP4表达下调对K1细胞生长及迁移的影响。方法利用慢病毒载体构建EP4沉默K1细胞株,qRT-PCR及Western blot法检测转染后K1细胞EP4 mRNA及蛋白表达;CCK8法和流式细胞术检测细胞生长及凋亡;transwell法检测细胞迁移。结果与阴性对照组相比,EP4基因沉默后K1细胞EP4 mRNA和蛋白表达明显下调,细胞生长受到明显抑制及细胞凋亡率显著增加。同时EP4沉默的K1细胞迁移能力显著降低。结论 EP4沉默可显著抑制K1细胞生长及诱导其细胞凋亡,并降低其细胞迁移能力。

Objective To investigate the effect of EP4 gene silencing on the growth and migration of K1 cells. Methods K1 cells with stable knockdown of EP4 were constructed with lentiviral vector. QRT-PCR and western blot analysis were used to detect the expression of EP4 mRNA and protein in K1 cells. CCK8 assay and flow eytometry were employed to measure cell viability and apoptosis. Transwell assay was applied to detect cell migration. Results Compared with the negative control group, the mRNA and protein expression of EP4 were sig- nificantly decreased in K1 ceils with stable knockdown of EP4. Furthermore, shRNA-mediated silencing of EP4 gene remarkably suppressed cell viability and induced apoptosis of K1 cells. The migration of K1 cells with knock- down of EP4 was decreased compared with the negative control group. Conclusions EP4 gene silencing can in- hibit growth and induce apoptosis of K1 cells. Downregulation of EP4 can significantly reduce migration of K1 cells.