尿毒清颗粒对慢性肾衰竭腹膜透析大鼠生存质量及TGF-β1/Smad通路表达的影响

Effects of Niaoduqing Granule on quality of life and TGF-β1/Smad signalling in chronic renal failure rats received peritoneal dialysis

目的研究尿毒清颗粒对慢性肾衰竭腹膜透析大鼠生存质量及TGF-β1/Smad通路表达影响。方法 30只SD大鼠构建腹膜透析模型,随机分为模型组(M组)、低剂量组(L组)、高剂量组(H组)。L、H组给药剂量分别为2、4 g/(kg·d)。观察大鼠体质量、自主活动次数、肾脏肥大指数、Scr、BUN、TGF-β1、p-Samd2/3。结果 (1)体质量增长速度:N组>H组>L组>M组;(2)M组自主活动次数减少最快,与其他组差异明显;(3)4组Scr、BUN、肾脏肥大指数比较,差异有显著性;L组、H组Scr、BUN、肾脏肥大指数、TGF-β1、p-Samd2/3与M组比较,均显著降低,L组、H组TGF-β1、p-Samd2/3比较,差异有显著性。结论尿毒清颗粒可提高慢性肾衰竭腹膜透析大鼠生存质量,降低Scr、BUN水平,调节机制可能与TGF-β1/Smad通路有关。

Objective To investigate the effect of Niaoduqing Granule on the quality of life and the activation of TGF-β1/Smad signalling in chronic renal failure rats received peritoneal dialysis. Methods Thirty SD rats were used to establish peritoneal dialysis models and were randomly divided into the model group （group M ）, the low dose group （group L）, and the high dose group （group H）. The dosage of group L and group H was 2 and 4 g/ （kg· d） , respectively. The body mass, the number of independent activities, the index of renal hypertrophy, Scr, BUN, experssion of TGF- β1 and p-Samd2/3 were determined. Results The body weight growth rate was shown decreased as follows ： group N 〉 group H 〉 group L 〉 group M. The number of spontaneous activity of group M decreased significantly than those in other groups. Scr, BUN and kidney hypertrophy index were significantly different among the four groups. Compared with group M, BUN, Scr, kidney hypertrophy index, levels of TGF- β1 and p-Samd2/3 were significantly decreased in group L and H. Levels of TGF-β1 and p-Samd2/3 were signifi- cant different between group L and H. Conclusions Niaoduqing Granule can improve the quality of life and re- duce the level of serum creatinine and urea nitrogen in chronic renal failure rats received peritoneal dialysis, and the mechanism may be associated with the regulation of TGF-β1/Smad signaling pathway.