FQs耐药基因aac(6′)-Ib-cr和qnrD及qepA三重PCR检测方法的建立及应用

Establishment and application of detection methods for FQs-resistance genes aac(6′)-Ib-cr,qnrD and qepA in bacteria by multi-PCR

为更好地指导临床用药，本试验利用三重PCR反应体系对aac（6′）-Ib—cr、qnrD和qepA这3个耐药基因进行了检测，成功建立了一种快速、准确的细菌耐药性检测方法。结果显示，本试验建立的多重PCR技术与单重PCR的符合率达到90％以上，特异性高，重复性好，敏感性最大可以达到10叫稀释浓度，不仅为大量临床样品的检测节约了时间和经济成本，也为检测大肠杆茵对氟喹诺酮类药物的耐药基因提供了新的技术手段。

To provide an instruction for clinical medication,this experiment was carried out by using triple PCR reaction system and to detect aac （6′）-Ib-cr,qnrDand qepAresistance genes,and succes- sfully established a rapid and accurate detection method of bacterial resistance. The results showed that the established multiplex PCR technique and single PCR experimental coincidence rate reached more than 90%.Moreover, the multiplex PCR＇s specificity is high,and repeatability is good. lt＇s sensitiyew ben the samples were diluted by 104 times,which not only saves time and cost for a large number of clinical samples, but also provide a new technique for detection of Escherichia coli fluoroquinolone resistance gene.