高效液相色谱法测定血浆中氯胺酮浓度及其药物动力学参数

Determination of ketamin concentration in blood Plasma by HPLC and pharmacomkinetic parameters

本文采用反相高效液相色谱法以布比卡因为内标测定氯胺酮的血药浓度。该法回收率为101.80%,变异系数为2.71%,?内精密度CV为1.49%—4.95%。测定了受手术者(inf)药时数据,其半衰期分别为t_(1/2)α=0.1141±0.0437h,t_(1/2)S=1.9030±0.5617h。并对实验结果临床意义进行了讨论、

A reversed-phase HPLC method for determination of ketamine concentrationin human blood plasma was reported. bupivacaine was used as the inner standard.the average recoveries were 101.8% The coefficient of variation was 2. 71%. the precis-ions of within the same day and in diff erentday CV were 1.49%-4.95%. ketamineconcentration-Time data of patients operated (inf. ) wer determined. the half-lifewas: t1/2 α=0. 1141±0.0437h t1/2β=1.903±0.5617h, respectively. clinical values ofthe experimental result were discussed.