革兰染色在真菌菌丝鉴别中的价值

Significance of Gram's staining method in identification of fungal hyphae

目的探讨痰涂片革兰染色在真菌的真假菌丝鉴别中的价值。方法对2016年1月—2017年6月天津市宝坻区中医医院门诊及住院患者的1718例痰液标本进行痰涂片革兰染色,之后对痰涂片上发现真菌菌丝的370例痰液标本进行常规的真菌培养鉴定,观察和记录结果,并进行相关性分析。结果 1718例痰液标本中,有318例发现革兰阳性假菌丝,52例发现革兰阴性菌丝,其他标本未发现真菌菌丝;对370例痰液标本的培养鉴定结果显示,318例革兰阳性假菌丝痰液标本生长的菌落均为假丝酵母菌,52例革兰阴性菌丝痰液标本生长的菌落均为曲霉菌。结论痰涂片革兰染色可作为真菌的真假菌丝鉴别方法之一。

Objective To estimate the value of Gram＇s staining method used for sputum smear in the identification between true and false fungal hyphae. Methods From January 2016 to June 2017, the Gram＇s staining was made for 1718 sputum smear specimens collected from outpatients and inpatients in Baodi Chinese Medicine Hospital of Tianjin. The fungal hyphae were found in sputum smear specimens of 370 patients, then their sputum fungal cultures were done routinely to identify the true or pseudo hyphae, the observation situations and results were recorded, and the correlation analyses were carried out. Results Among the sputum smear samples of 1718 patients, the gram-positive pseudohyphae were found in 318 cases, gram-negative hyphae were shown in 52 cases, and no fungal hyphae were found in other smear samples; the 370 sputum culture identification results showed that growing fungal colonies in 318 specimens with gram-positive pseudohyphae were all belong to pseudohyphae candida, while the growing colonies in the 52 specimens with gram-negative hyphae were all belong to aspergillus. Conclusion The sputum smears of Gram stain can be used as one of the methods to identify the true or pseudo hyphae.