丙型肝炎病毒抗体检测联合核酸检测在血液筛查中的价值

Value of Hepatitis C Virus antibody detection combined with nucleic acid detection in blood screening

目的探讨丙型肝炎病毒抗体(HCV-Ab)检测联合核酸检测在丙型病毒性肝炎(丙肝)血液筛查中的价值。方法选择2017年1月—12月福建医科大学附属宁德市医院进行HCV-Ab检测和核酸检测的3925份血液标本,统计单独HCV-Ab检测、单独核酸检测以及联合两种方法检测的阳性率的差别。结果单独进行HCV-Ab检测结果显示阳性2例,不确定1例;单独进行核酸检测未见阳性结果;而HCV-Ab检测联合核酸检测的结果显示,3925份血液标本中有9份呈阳性,检出率为0.23%。可见联合检测的阳性检出率明显高于单独进行HCV-Ab检测和核酸检测的阳性检出率(P<0.05)。结论血液筛查中应用HCV-Ab检测与核酸检测联合的方式能够更加准确地诊断相关疾病,更好地保证血液安全。

Objective To explore the value of hypatitis C virus antibody（HCV-Ab） detection combined with nucleic acid detection in the blood screening of hepatitis C virus（HCV）. Methods From January to December 2017, 3 925 blood specimens for HCV-Ab and nucleic acid testing were collected from Ningde Municipal Hospital Affiliated to Fujian Medical University. The differences among the positive rates of the single HCV-Ab test, the single nucleic acid test and combination of the two methods were statistically analyzed. Results The results of HCV-Ab detection alone showed that 2 cases were positive and 1 case was uncertain; no positive results were obtained by nucleic acid detection alone; however, the results of HCV-Ab detection combined with nucleic acid detection showed that 9 of the 3 925 blood samples were positive and the detection rate was 0.23%. Therefore, the positive rate of combined detection was obviously higher than that of either HCV-Ab or nucleic acid detection alone（P 〈0.05）. Conclusion The combination of HCV-Ab detection and nucleic acid detection in blood screening can make a more accurate diagnosis of related diseases and better ensure the safety of blood.