玉屏苍耳散对过敏性鼻炎模型大鼠免疫调节的影响

Yuping-Canger powder regulation on allergic rhinitis in rats with immune function

目的 探讨玉屏苍耳散对过敏性鼻炎（allergic rhinitis, AR）模型大鼠免疫调节的作用。方法 将80只雌性SD大鼠按随机数字表法分为模型组、低剂量组、高剂量组、空白组，每组20只。采用卵清蛋白致敏及激发建立AR大鼠模型，同时低剂量组、高剂量组分别采用微量加样器滴于鼻孔给予玉屏苍耳散（1.0、4.0 g/kg），模型组和空白组等体积给予生理盐水，处理8周。采用ELISA法测定血清卵清蛋白-特异性免疫球蛋白E（ovalbumin special immunoglobulin E, OVA-sIgE）水平、IL-4、IL-10和IFN-γ水平，采用流式细胞术检测脾细胞悬液中CD4＋CD25＋T细胞比例。结果 与模型组比较，低剂量组、高剂量组的IL-4含量[（62.9±10.5）ng/ml、（69.2±10.7）ng/ml比（74.9±11.2）ng/ml]、IL-10含量[（75.6±10.8）pg/ml、（70.9±10.4）pg/ml比（168.6±15.2）pg/ml]、OVA-sIgE含量[（7.2±0.5）ng/ml、（5.1±0.4）ng/ml比（24.9±2.6）ng/ml]降低（P＜0.05），CD4＋CD25＋T细胞比例[（29.9±10.5）%、（31.2±10.7）%比（14.9±11.2）%]增加（P＜0.05）。结论 玉屏苍耳散可抑制Th2细胞反应，增强 CD4＋CD25＋T细胞活性，发挥抗过敏作用。

Objective To study the regulation of Yuping-Canger powder on allergic rhinitis in rats with immune function. Methods 80 female SD rats were randomly divided into 4 groups： respectively, model group, low dose group, high dose group and control group. 20 rats in each group. Used ovalbumin （OVA） sensitization and stimulate to establish the rat model of allergic rhinitis, and low dose group, high dose group were given Yuping-Canger powder （1 g/kg and 4 g/kg） treatment, model group and control group were given normal saline, treatment for 8 weeks. The serum OVA specific IgE by ELISA （OVA-sIgE）, IL-4, IL-10 and IFN-γ levels, spleen cells was detected by flow cytometry. Spleen cells detected by flow cytometry CD4＋CD25＋T cell ratio in liquid suspension. Results Compared with the control group, IL-4, IL-10 and IFN-γ, OVA-sIgE, CD4＋CD25＋T cells of model group, low dose group, high dose group increased significantly （P〈0.05）; compared with the model group, of IL-4, IL-10 and OVA-sIgE of low dose group, high dose group decreased, the proportion of CD4＋CD25＋T cells increased significantly （P〈0.05）; compared with the low dose group, IL-4, IL-10 and OVA-sIgE of high dose group decreased significantly, the proportion of CD4＋CD25＋T cells increased significantly （P〈0.05）. Conclusions The Yuping-Canger powder can inhibit Th2 cell responses and enhanced CD4＋CD25＋T cell activity play anti allergic effect, and thus play a role in the treatment of allergic rhinitis.