液相色谱-质谱法测定金银花中噻菌灵的残留量

Determination of thiabendazole in honeysuckle flower by LC-MS/MS

目的 建立液相色谱-质谱法（liquid chromatography-mass spectrometry, LC-MS/MS）测定金银花中噻菌灵残留量的方法。方法 样品采用乙酸乙酯提取，使用LC-MS/MS法测定金银花中噻菌灵含量，在正离子模式下，采用选择离子监测方式，其监测离子对为m/z 202→175。结果 在0.1×10-5～2×10-5 mg范围内噻菌灵获得良好线性关系（r＝0.999 5），检测限为10.0 ng/ml，平均回收率为93.70%，RSD为2.53%。结论 所建立的LC-MS/MS法快速灵敏，适用于金银花中噻菌灵残留量的检测。

Objective To establish a rapid and sensitive liquid chromatograph-mass spectrometry （LC-MS/MS） method for the determination of thiabendazole in honeysuckle flowers. Methods The acetic ether was selected as extraction solvent. The mass spectrometer analysis was conducted in the positive ionization electrospray mode using SIM. The transitions m/z 202→175 was used to quantify thiabendazole. Results The satisfactory linearity was obtained in the range of 0.1×10-5-2×10-5 mg for thiabendazole （r=0.999 5）, and the limit of detection （LOD） of 10.0 ng/ml and the mean recovery of 93.87% were obtained by this LC-MS/MS method. Conclusions The method of LC-MS/MS is sensitive, simple and accurate, and it proved to be suitable for the determination of thiabendazole in Honeysuckle flowers.