Mg^(2+)依赖性蛋白磷酸酶1δ的生物信息学分析

Bioinformatics analysis of protein phosphatase magnesium-dependent 1δ

Mg2+依赖性蛋白磷酸酶1δ(protein phosphatase magnesium-dependent 1δ,PPM1D)作为肝癌潜在的预后标志物和治疗靶点,其致癌机制和预后价值仍未完全阐明。为了全面认识PPM1D,使用生物信息学方法,对PPM1D蛋白的序列同源性、组织表达、亚细胞定位、理化性质、空间结构及蛋白质相互作用网络进行分析。结果表明:人PPM1D基因编码605个氨基酸组成的多肽,与物种进化程度一致,属于PP2C蛋白超家族,是碱性不稳定的亲水蛋白,无信号肽和跨膜区域;PPM1D蛋白主要定位于细胞核内,其主要二级结构为随机卷曲,存在磷酸化、乙酰化、甲基化和泛素化位点,与PPM1D相互作用的蛋白主要是细胞周期检查点蛋白和细胞损伤修复相关蛋白。根据分析结果阐述了PPM1D蛋白与癌症的相关性以及PPM1D蛋白作为癌症标志物的理论基础,为进一步研究该蛋白及其参与的信号通路提供一定的借鉴和参考。

Protein phosphatase magnesium-dependent 1δ（ PPM1 D） is a potential prognostic marker and therapeutic target for hepatocellular carcinoma. But its carcinogenic mechanism and prognostic value have not been fully elucidated. To gain insightful information of PPM1 D protein,bioinformatics methods are applied to analyze the hereditary conservation, tissue expression, subcellular localization, chemical properties, space structure and protein interaction networks of PPM1 D protein. The PPM1 D protein is comprised of 605 amino acid residues which belong to the PP2 C super family. It is a hydrophilic unstable protein without signal peptide and trans-membrane region. It is mainly located in the nucleus and the main secondary structure elements are random coil. It contains several phosphorylation,acetylation,methylation and ubiquitination sites. Interactive proteins with PPM1 D are mainly cell cycle checkpoint proteins and cell damage and repair-related proteins. We analyze the relationship between PPM1 D protein and cancer and the theoretical basis of PPM1 D as the cancer marker,which provide some references for further study of the protein and its participation in signal pathways.