鼻咽清颗粒对鼻咽癌细胞裸鼠移植瘤的放疗增敏作用及机制研究

Radiosensitization of Bi-Yan-Qing Granules on human nasopharyngeal planted tumor and its mechanism

目的体内考察鼻咽清颗粒对人鼻咽癌细胞(CNE-2)裸鼠种植瘤的放疗增敏作用并对其可能机制进行初步探索。方法建立CNE-2细胞的裸鼠种植瘤模型,取荷瘤成功裸鼠30只,随机分成5组,分别为模型组,鼻咽清颗粒低、高剂量(生药剂量5.2、10.4 g/kg)组,放疗组,鼻咽清颗粒(生药剂量5.2 g/kg)+放疗组,每天2次ig给药;放疗照射剂量为4 GY,隔日1次,连续2周。给药期间每5天测量肿瘤体积,至给药30 d处死,取瘤组织,称质量并计算抑瘤率;免疫组化检测P53、Bcl-2蛋白表达。结果与模型组比较,各治疗组均对移植瘤体积、质量发挥显著抑制作用(P<0.05、0.01);鼻咽清颗粒+放疗组瘤体积、质量均显著低于放疗组(P<0.05、0.01)。免疫组化结果提示,与模型组比较,各治疗组移植瘤组织中P53、Bcl-2蛋白表达阳性细胞率均显著降低(P<0.05、0.01);与放疗组比较,鼻咽清颗粒+放疗组P53、Bcl-2蛋白表达阳性细胞率显著降低(P<0.01)。结论鼻咽清颗粒抑制鼻咽癌的生长且具有放疗增敏作用,可能与同时抑制突变性P53和Bcl-2蛋白表达相关。

Objectives To study the radiosensitization effect of Bi-Yan-Qing granules on xenograft tumor of human nasopharyngeal carcinoma cell line CNE-2 in the BALB/C nude mice and explore the possible mechanism. Methods CNE2 cells were injected subcutaneously into nude mice to establish transplanted tumor mice model, Then, 30 mice were randomly divided into five groups： model group, Bi-Yan-Qing Granules low and high dosage（crude drug dosage： 5.2, 10.4 g/kg） groups, irradiation group, and Bi-Yan-Qing Granules（crude drug dosage： 5.2 g/kg） combined with irradiation group. Ig administered for twice a day, radiotherapy dose was 4 GY, once two days for two weeks. The tumor volume was measured every five days during dosing period. Nude mice were killed after 30 days administration, and the tumor tissue was weighed to calculate tumor inhibition rate. The immunohistochemistry assay was used to detect the expression levels of P53 and Bcl-2 protein in tumor tissues in each group. Results Compared with model group, the transplantation tumor volume and weight of each treatment group were significantly inhibited（P 0.05, 0.01）. The tumor volume and quality of combination group（Bi-Yan-Qing Granules and irradiation） were significantly lower than those of the radiotherapy group（P 〈0.05, 0.01）. Immunohistochemistry results showed that, compared with model group, the positive cellular rate of P53 and Bcl-2 protein expression were significantly decreased in tumor tissue of each treatment group（P 〈0.05, 0.01）. Compared with radiotherapy group, the positive cellular rate of P53 and Bcl-2 protein expression in the combination group was significantly reduced（P 〈0.01）. Conclusions Bi-Yan-Qing Granules inhibit the growth of nasopharyngeal carcinoma and have a radiosensitization effect, which may be related to the inhibition of mutation in P53 and Bcl-2 protein expression at the same time.