摘要
本文主要研究了脉冲电场对酵母菌细胞形态的影响,酿酒酵母(Saccharomyces cerevisiae CICC 1012)菌悬液在脉冲电场条件下进行处理,采用台盼蓝染色和扫描电镜观察发现,酵母菌细胞的表面粗糙度明显增强,甚至出现未复原的小凹孔.该项研究可为外源蛋白质、药物等大分子的导入提供技术支持.
Effects of pulse electric field on yeast Saccharomyces cerevisiae CICC 1012 cellular morphology was determined. Pulse electric field treatments increased cell roughness and caused some small holes as visualized through trypan blue staining and scanning electron microscopy. This provides insights for insertion of foreign proteins or medicinal molecules into yeast cells.
作者
袁晓东
朱国立
YUAN Xiao-dong1, ZHU Guo-li1,2(1.College of Agriculture Inner Mongolia University for Nationalities, Tongliao 028043, China; 2. Tongliao Academy of Agricultural Sciences, Tongliao 028000, Chin)
出处
《内蒙古民族大学学报（自然科学版）》
2017年第2期143-146,共4页
Journal of Inner Mongolia Minzu University：Natural Sciences
基金
国家自然科学基金项目(3150000)
内蒙古民族大学科学研究基金资助项目(NMDYB15015)
关键词
脉冲电场
酵母菌
细胞形态
Pulse electric field
Yeast
Cellular morphology
