支气管哮喘急性发作患者血清中白细胞介素-18、白细胞介素-33变化及临床意义

The expression and clinical significance of interleukin -18 and interleukin -33 in serum of patientswith acute asthma attack

目的 通过观察支气管哮喘急性发作患者血清中白细胞介素（IL）-33、IL-18水平,探讨IL-33、IL-18表达水平与支气管哮喘急性发作之间的相关性.方法 选取80例支气管哮喘急性发作患者（观察组）和80例同期健康体检者（对照组）,采用酶联免疫吸附法（ELISA）测定两组血清IL-33和IL-18水平,并进行比较.采用全细胞自动分析仪和免疫比浊法测定观察组患者血清中嗜酸性粒细胞（EOS）和免疫球蛋白E（IgE）水平,比较治疗前后EOS、IgE水平与IL-33和IL-18水平的相关性.结果 观察组治疗前IL-18水平为（310.2±152.6）ng/L,IL-33水平为（215.4±87.5）ng/L,与健康组[（204.2±95.4）、（138.7±48.3）ng/L]比较差异有统计学意义（P〈0.05）.观察组治疗后血清IL-18水平为（215.5±98.2）ng/L,IL-33水平为（146.6±54.9）ng/L,与治疗前比较差异有统计学意义（P〈0.05）.观察组治疗前后EOS和IgE水平与IL-18和IL-33水平呈正相关（P〈0.05）.结论 哮喘急性发作患者血清IL-18与IL-33水平升高,治疗后两者水平均明显下降.两者与EOS和IgE水平呈正相关.

Objective To analyze the relationship between serum levels of interleukin （IL）-33 , IL-18 and the level of immunoglobulin-E （IgE）, eosinophils （EOS） in patients with acute asthma attack. Methods Eighty patients of acute asthma attack （observation group） and 80 cases of healthy physical examination （control group） were selected. The levels of IL-33 and IL-18 were determined by enzyme-linked immunosorbent assay （ELISA） method and compared between two groups. The levels of EOS and IgE were measured by automatic analyzer , and the relationship between serum levels of interleukin （IL）-33 and IL-18 and the level of immunoglobulin-E （IgE） and eosinophils （EOS） was analyzed in patients with acute asthma attack. Results The levels of IL-18 and IL-33 in observation group were （310.2 ± 152.6）, （215.4 ± 87.5） ng/L, in control group were（204.2 ± 95.4）, （138.7 ±48.3） ng/L, and there were significant differences （P 〈 0.05）. The levels of IL-18 and IL-33 in observation group after treatment were （215.5 ± 98.2）, （146.6 ± 54.9） ng/L, significantly lower than those before treatment （P〈0.05）. The levels of EOS and IgE had positive relation with the level of IgE and EOS （P 〈 0.05）. Conclusions The levels of IL-18 and IL-33 in patients with acute asthma attack increase, and both of them significantly decrease after treatment. They are positively correlated with IgE and EOS.