臭氧、电子束辐照降解玉米赤霉烯酮和赭曲霉毒素A

Study of ozone and electron beam irradiation on degradation of zearalenone and ochratoxin A

玉米赤霉烯酮(Zearalenone,ZEN)和赭曲霉毒素A(Ochratoxin A,OTA)毒性较大、污染范围较广,一直是中国食品行业重点关注的问题。采用毒素标准品,分别考察臭氧和电子束辐照对其降解效果。研究结果表明,2 mL50μg/mL的ZEN经2.0mg/L的臭氧处理10s后,ZEN未检出;经12kGy的电子束辐照后,降解率达86%,且0.5~5.0μg/mL时,ZEN浓度对其降解效果无显著影响(P<0.05),ZEN在乙腈中较甲醇降解更快。2 mL 5μg/mL的OTA经50mg/L的臭氧处理30s后,OTA降解率为22%,当处理时间延长至180s,降解率仍无显著提高;0.1~1.0μg/mL浓度的OTA经12kGy剂量电子束辐照后,降解率均在90%以上,且OTA浓度对其降解率无显著影响(P<0.05),OTA在乙腈中较甲醇更快降解。臭氧较电子束易降解ZEN,电子束较臭氧更易降解OTA。研究结果为臭氧和电子束辐照降解不同真菌毒素提供了理论参考和实践依据。

Mycotoxin contamination caused by Zearalenone （ZEN） and Ochratoxin A （（）TA） is widespread for being prone to happen and hard to degrade. In this paper, the effects of ozone and electron beam irradiation （EBI） on the degradation of Zearalenone and Ochra toxin A were investigated. After 10 s of 2.0 mg/L ozone treatment, the ZEN content decreased from 50 μg/mL to concentration unde tectable. After 12 kGy of EBI, the degradation rate of ZEN was up to 86% and concentration of ZEN within 0.5-5.0 μg/mL had no signif icant different （P〈0.05）, with the degradation of ZEN being faster in acetonitrile than methanol. After 30 s of 50 mg/mL ozone treat ment, the degradation rate of 5μg/mL OTA was up to 22%, with no significant improvement of 180 s treatment. After 12 kGy of EBI, the degradation rate of OTA was up to 90% and the concentration of OTA had no significant different （P〈0.05）, with the degradation nf OTA being faster in acetonitrile than methanol. In general, the deg radation of ZEN by ozone was easier than EBI, with EBI being easier than ozone for the degradation of OTA. In this study, a comprehen sive study of the application of ozone and EBI on degradation of ZEN and OTA was intended for providing theoretical and practical reference.