钩藤茎枝不同药用部位总生物碱的含量分析

Analysis on the content of total alkaloids in different parts of uncaria stems branche for medicine

目的比较钩藤茎枝药典规定药用部位和超出部位总生物碱的含量差异。方法以钩藤碱为对照品,采用紫外分光光度法在检测波长243 nm处测定10批钩藤中不同用药部位总生物碱的含量。结果钩藤碱的浓度在2~12μg/ml(r=0.9996)范围内与吸光度呈良好的线性关系,加样回收率为103.30%,RSD为2.60%。结论该方法简单,准确性、重复性良好,为钩藤药材的净选提供基础性研究。

Objective To compare the differences in total alkaloids contents between the stems branches of uncaria limited by the pharmacopeia and the parts beyond the limitation. Methods Rhynchophylline was used as a reference substance,and the ultraviolet spectrophotometry was used to determine the contents of total alkaloids in 10 batches of different parts of uncaria by UV at 243 nm. Results The concentration of rhynchophylline in 2 - 12 μg/ml（ r = 0. 9996） showed a good linear relationship between absorbance range. The average recovery was 103. 30%,with RSD = 2. 60%. Conclusion The method is simple,sensitive and accurate,which can offer a basic research for the selecting uncaria medicine.