高良姜提取物不同组分的抗氧化活性比较

Comparison of antioxidant activity in different components of Alpinia Officinarum extract

目的比较高良姜提取物不同组分的抗氧化活性。方法采用减压液相层析法(VLC)分离高良姜提取物,再用高效液相色谱法(HPLC)和液相色谱-质谱联用法(LC-MS)进行归属鉴别。应用H_2O_2诱导人肝癌细胞株HepG2氧化损伤模型及小鼠负重游泳实验,MTT法和流式细胞术检测不同组分高良姜提取物的抗氧化活性。结果高良姜提取物被分离出A^G等7个组分,归类为二苯基庚烷类(A、B)、黄酮类(C、D)、挥发油类(E、F、G)。组分A、B、D和F提高氧化损伤HepG2细胞存活率(P<0.05),而组分B、D降低氧化损伤HepG2细胞凋亡率(P<0.05)。低剂量组二苯基庚烷类显著延长小鼠负重游泳时间(P<0.05)。结论高良姜提取物中二苯基庚烷类具有显著的抗氧化活性。

Objective To compare the antioxidant activity in different components of Alpinia Officinarum extract.Methods The galangal extracts were separated by vacuum liquid chromatography（VLC）, and then identified by HPLC and LC-MS. The antioxidant activity of different components of galangal extracts was determined by MTT assay and flow cytometry in H2O2-induced oxidative damage model of HepG2 cells and weight loading swimming mice. Results The galangal extracts were separated into A-G components, belonging to diphenylheptanes（A, B）, flavonoids（C, D） and volatile oils（E, F, G）. The components A, B, D and F increased the survival rates of oxidative damage HepG2 cells（P〈0.05）, while components B and D decreased the apoptosis rates（P〈0.05）. The low dose of diphenylheptanes enhanced the weight loading swimming time of the mice（P〈0.05）. Conclusion Diphenylheptanes of galangal extracts possess the antioxidant activity.