经前期综合征患者外周血CD8^+CD28^-T细胞、TGF-β1、IL-1水平检测及其与心理应激的关系

Relationship between mental stress and peripheral blood levels of CD8^+CD28^-T cells, TGF-β1 and IL-10 in patients with premenstrual syndrome

目的探索经前期综合征(PMS)患者心理应激与CD8^+CD28^-T细胞及其相关细胞因子的关系。方法选取50例PMS患者(PMS组)、35例单纯焦虑患者(单纯焦虑组)及30例健康女性(健康组),采用流式细胞术检测外周血CD8^+CD28^-T细胞、TGF-β1及IL-10水平,焦虑自评量表(SAS)和抑郁自评量表(SDS)检测心理应激程度,并观察阿普唑仑或维生素B6治疗后变化。结果与健康组比较,PMS组和单纯焦虑组CD8^+CD28^-T细胞、TGF-β1水平降低(P<0.01或0.05),尤以PMS组更显著。PMS患者CD8^+CD28^-T细胞、TGF-β1水平与SAS评分呈显著负相关(P<0.01)。PMS患者在阿普唑仑或维生素B6治疗后CD8^+CD28^-T细胞、TGF-β1及IL-10水平均较治疗前回升(P<0.01)。结论 PMS患者的焦虑状态与CD8^+CD28^-T细胞、TGF-β1水平降低有关。

Objective To investigate the relationship between mental stress and levels of CD8~＋CD28^-T cells and related cytokines in patients with premenstrual syndrome（PMS）. Methods Fifty patients with PMS, 35 cases of pure anxiety and 30 healthy women were enrolled. The mental stress was evaluated by self-rating anxiety scale（SAS） and self-rating depression scale（SDS）. Peripheral blood levels of CD8~＋CD28^-T cells, TGF-β1 and IL-10 were detected by flow cytometry before and after alprazolam or vitamin B6 treatment. Results Compared with healthy group, CD8~＋CD28^-T cells and TGF-β1 levels were decreased in PMS group and pure anxiety group（P〈0.01 or 0.05）, especially in PMS group. CD8~＋CD28^-T cells and TGF-β1 levels were negatively correlated with SAS scores in PMS group（P〈0.01）. After treatment with alprazolam or vitamin B6,levels of CD8~＋CD28^-T cells, TGF-β1 and IL-10 were increased（P〈0.01）. Conclusion The anxiety state is associated with downexpression of CD8~＋CD28^-T cells and TGF-β1 in patients with PMS.