摘要
目的探讨HIF-1α与TREM-1在寻常性银屑病发病中可能的作用机制。方法采用免疫组织化学与实时荧光定量PCR的方法检测缺氧诱导因子1α(HIF-1α)与髓样细胞触发受体-1(TREM-1)在寻常性银屑病与正常皮肤组织中的表达情况,并分析HIF-1α与TREM-1在银屑病组织中表达的相关关系。结果正常皮肤组织中HIF-1α与TREM-1主要表达于基底细胞层,在寻常性银屑病患者皮损中HIF-1α与TREM-1表达增加,主要表达于表皮全层,其表达水平差异具有统计学意义(P<0.05)。HIF-1αmRNA与TREM-1 mRNA在寻常性银屑病皮损表达均升高,与正常组织相比,差异具有统计学意义(P<0.05)。并且HIF-1α与TREM-1在寻常性银屑病组织中表达呈正相关(P<0.05)。结论 HIF-1α与TREM-1在寻常性银屑病的异常表达可能参与了寻常性银屑病发生、发展,并在此过程中存在共同路径,TREM-1可能成为治疗寻常性银屑病的潜在靶点。
Objective To discuss potential pathogenesis of HIF-1α and TREM-1 in psoriasis.Methods Immunohischemistry and realtime-PCR were used to detect expression of HIF-1α and TREM-1 in normal skins and psoriasis lesions,and the relationship between TREM-1 and HIF-1α in psoriasis were analyzed.Results The expression of HIF-1α and TREM-1 were limited to stratum basele in normal skin,while they expressed in the psoriasis lesions,however, in the whole epidermis.The expression levels of HIF-1α and TREM-1 in psoriatic lesions were significantly higher than that in normal tissues(P〈0.05).Moreover,the expression of HIF-1α was positively correlated with the expression of TREM-1 in psoriasis vulgaris(P〈0.05).Conclusion The higher expression of HIF-1α and TREM-1 which participate in the same mechanism may play an important role in pathogenesis of psoriasis.Therefore, TREM-1 will be the potential therapeutic target of psoriasis.
作者
涂晨
王爽
王晓鹏
胡啸
任建文
肖生祥
TU Chen, WANG Shuang, WANG Xiaopeng, HU Xiao, REN Jianwen, XIAO Shengxiang(Department of Dermatology, the Second Hospital of Xi'an Jiaotong University,Xi'an 710004, Chin)
出处
《中国皮肤性病学杂志》
CAS
CSCD
北大核心
2018年第4期391-395,共5页
The Chinese Journal of Dermatovenereology