痛泻要方对IBS肝郁脾虚证大鼠血清、结肠5-羟色胺的影响

Effect of on serum and colon 5-serotonin in rats with irritable bowel syndrome liver-depression and spleen-deficiency

目的:探讨痛泻要方对肠易激综合征肝郁脾虚证大鼠血清、结肠5-羟色胺(5-HT)的调控。方法:将120只SPF级SD大鼠随机分为6组:正常组、病理组、痛泻要方低剂量组、痛泻要方中剂量组、痛泻要方高剂量组、西药(匹维溴铵)组,每组20只。除正常组外,其余各组均用"束缚应激+番泻叶灌胃法"进行造模,前肢束缚2 w,在束缚的基础上进行番泻叶灌胃1 w,1次/d。后用相应剂量的痛泻要方和匹维溴铵进行灌胃治疗7 d。造模及治疗结束后,检测各组大鼠血清及结肠5-HT的含量。结果:与正常组相比,模型组大鼠血清、结肠的5-HT含量显著升高(P<0.01);与模型组相比,痛泻要方低、中、高剂量组和西药组大鼠血清、结肠的5-HT含量明显降低(P<0.05或P<0.01),以痛泻要方高剂量组大鼠的5-HT含量最接近正常组水平。结论:痛泻要方通过疏肝、柔肝的作用,减少大鼠血清和结肠5-HT的含量,来缓解IBS肝郁脾虚证大鼠的症状。

Objective：To discuss effect of Tongxie Yaofang on Serum and Colon 5-serotonin in rats with Liver-depression and Spleen-deficiency irritable bowel syndrome. Method：120 SPF SD rats were divided into six groups with 20 rats in each group：normal group,pathological group,low,middle and high doses group of Tongxie Yaofang and piroxicam group. The model was made by restraint stress and Fanxieye gavage,forelimb restraint 2 weeks except the normal group. On the basis of restraint Sennae intragastric administration 1 weeks for once a day. Western medicine group was given pinaverium bromide,low,middle and high dose groups were given with Tongxie Yaofang of different dose. The contents of 5-HT in serum and colon in each group were detected. Results：Compared with the normal group,the content of 5-HT in the serum and colon of the model group was significantly increased（P〈0.01）. Compared with the model group,the contents of 5-HT in serum and colon of Tongxie Yaofang low,medium and high dose groups and the piroxicam group were significantly decreased（P〈0.05,P〈0.01）. The content of 5-HT in Tongxie Yaofang high dose group was the closest to that of normal group. Conclusion：Tongxie Yaofang can alleviate the symptoms of IBS with liver-depression and spleen deficiency syndrome by reducing the content of 5-HT in serumard colon.