丁苯酞注射液对原代培养神经元类缺血再灌注损伤的影响

Effect of butylphthalide and sodium chloride injection on primary cultured neuronal reperfusion injury

目的:探讨丁苯酞注射液对原代培养神经元类缺血再灌注损伤的影响。方法:原代培养大鼠神经元建造实验模型,并随机分为对照组、缺血组(类缺血后再灌注)、实验组(类缺血再灌注后加丁苯酞),造模后鉴定培养细胞为神经元,分组后检测神经元细胞活性、神经元细胞凋亡率。结果:与对照组相比,缺血组的细胞存活率下降,细胞凋亡率增高。实验组细胞存活率高于缺血组,而细胞凋亡率低于缺血组。结论:丁苯酞注射液可显著增加类缺血再灌注神经元细胞再灌注后细胞的存活率,这种神经保护机制在一定程度上是通过其减少神经细胞凋亡而实现的。

Objective：To investigate the effect of butylphthalide and sodium chloride injection on primary ischemia reperfusion injury in neurons. Methods：Primary culture of rat neurons was established and was randomly divided into control group,ischemia group（ischemia-reperfusion）,experimental group（after ischemia-reperfusion plus butylphthalide）. The morphology of neurons was compared by MTT colonmetry. The apoptotic rate of neurons was measured by flow cytometry.Results：Compared with the control group,the cell survival rate of the ischemic group was decreased,and the apoptosis rate was increased. The cell survival rate of experimental group was higher than that of ischemic group,but the rate of apoptosis was lower than that of ischemic group. Conclusion：Butylphthalide and sodium chloride injection can significantly increase the survival rate of neuronal cells after ischemia-like-reperfusion injury,and this neuroprotection mechanism is related to its reduction of nerve cell apoptosis in some extent.