摘要
L-色氨酸表达异常与多种疾病相关,我们建立一种方法检测尿液中色氨酸含量。首先通过SCX强阳离子交换剂对尿液进行前处理,回收率为78.2%,再利用埃利希氏反应对尿液色氨酸进行检测,采用紫外-可见分光光度计测定595nm处吸收值,在0-200μM范围内呈线性,线性相关系数达到0.9928。本研究实现了尿液中色氨酸的简单、快速、可视化检测。
The abnormal expression of L-tryptophan is associated with a variety of diseases. We have established a method to detect the content of tryptophan in urine. First, the pretreatment of urine was carried out through SCX strong cation exchanger. The recovery rate was 78.2%. Then the Ehrlich＇S reaction was used to detect the tryptophan in urine. The absorption value of 595 nm was measured by UV-Vis spectrophotometer. It was linear in the range of 0-200 μM, and the linear correlation coefficient reached 0.9928. The simple, rapid and visual detection of tryptophan in urine was realized.
作者
李婷婷
刘若男
顾月清
Li Tingting, Liu Ruonan, Gu Yueqing(1.School of Engineering, China Pharmaceutical University, Nanjing, Jiangsu 210009, Chin)
出处
《生命科学仪器》
2018年第1期43-47,37,共6页
Life Science Instruments
基金
国家自然科学基金81727804
