一起副溶血性弧菌引起食物中毒的病原学鉴定及分型

Pathogenic and Typing Identification for A Foodborne Vibrio Parahemolyticus Caused Outbreak

目的对食物中毒事件中所采集样本进行副溶血性弧菌的分离鉴定,快速查明食物中毒原因并进行同源性分析。方法采用现行标准检验方法及实时荧光定量PCR对样本进行副溶血性弧菌分离鉴定;采用脉冲场凝胶电泳(PFGE)对分离到的副溶血性弧菌进行分子分型。结果运用标准检验方法在22份食物样本中分离到副溶血性弧菌1株,7份粪便样本中分离到5株;实时荧光定量PCR在食物样本中检出核酸阳性1份,粪便样本中检出6份;所分离到的6株副溶血性弧菌经PFGE聚类分析得到4种PFGE带型,指纹图谱相似度为93.48%~97.44%。结论此次食物中毒由同一克隆副溶血性弧菌污染所致,PFGE可有效用于食物中毒的分型研究及溯源分析,实时荧光PCR可作为现行标准检验方法的有效补充。

Objective To investigate the cause of a foodborne outbreak, based on vibrio parahemolyticus （VP） isolation and identification and homology analysis. Methods VP was isolated and identified from the samples by current standard test methods and real - time fluorogenic quantitative PCR. Molecular typing of these isolated clones was based on pulsed field gel electrophoresis （PFGE）. Results There were 22 food and 7 stool samples collected, 1 VP clones from the food samples and 5 clones from the stool samples were isolated by standard test methods. 1 positive sample from food and 6 positive samples from stool for VP DNAwere determined by fluorogenic quantitative PCR. The isolated 6 clones were presented 4 PFGE banding pattern, with the similarity of fingerprints 93.48% to 97.44%. Conclusion This foodborne outbreak was cause by contamination of a single clone of VP. PFGE was an effective typing and tracing method for foodborne outbreak investigation. Real -time fluorogenic quantitative PCR may be a supplement methods for current standard test methods.