摘要
目的从人参茎叶中提取分离人参皂苷F2、Rg1、Rb1、Rb2、Rb3单体化合物,纯度达98%以上。方法从人参茎叶中提取总皂苷后,粗分段,经硅胶柱色谱法分离,再经加压C18反相柱色谱纯化。结果从人参茎叶中提取分离得到纯度达98%以上的目标人参皂苷。结论此方法简单可行,相较于其他方法节省成本和时间。
Objective:To study separating and extracting Ginsenoside F2,Rg1,Rb1,Rb2,Rb3 from Ginseng stem and leaves.More than 98% purity. Method: After total saponins were extracted from Ginseng stem and leaves, subsection,they were separated by silica gel column chromatography,then purified by pressure C18 reversed phase column chromatography.Results: Separated and extracted 98% purity of Ginsenoside F2、Rg1、Rb1、Rb2、Rb3. Conclusion: The method is simple and feasible, and saves cost and time compared with other methods.
作者
王和宇
徐芳菲
王国明
李蕾
谢丽娟
郭畅冰
曹志强
WANG He-yu, XU Fang-fei,WANG Guo-ming, LI Lei, XIE Li-juan,GUO Chang-bing,CAO Zhi--qiang(Jilin Ginseng Research Institute, Changchun, 13003)
出处
《人参研究》
2018年第1期12-14,共3页
Ginseng Research
基金
吉林省行业科研专项经费
项目名称:"高效反相技术分离制备人参皂苷新方法的研究"
项目编号:吉财教函[2014]219号