PCR法检测宫颈癌细胞中人乳头瘤病毒

Detection of Human Papillomavirus in Cervical Carcinomas with PCR Technique

采用ＰＣＲ（聚合酶链反应）技术对宫颈癌细胞中ＨＰＶ（人乳头瘤病毒）和ＨＰＶ１６、１８型的感染状况分别进行了研究。在新鲜组织中，宫颈癌细胞内ＨＰＶ和ＨＰＶ１６、１８型的感染率分别达８０％和７５％。但石蜡标本中ＨＰＶ和ＨＰＶ１６、１８型的感染率则为３３．３％和２６．７％。说明宫颈癌细胞中ＨＰＶ的感染率极高，且主要系ＨＰＶ１６、１８型的感染。ＰＣＲ技术检测宫颈癌细胞中的ＨＰＶ方法简便快速，灵敏度高。可广泛用于流行病学调查和高危致癌ＨＰＶ感染妇女的追踪观察。

Human papillomavirus(HPV) and type 16.18 of HPV DNA in human cervical carcinoma have been detected with polymerase chain reaction.In fresh specimens the infection rate of HPV and HPV16.18 were 80% and 75% separately.However in paraffin embedded specimens the infection rate of HPV and HPV16.18 were 33 3% and 26 7% respectively.The results shows the infection rate of HPV in human cervical carcinoma is very high.Most of them were HPV 16.18 infection.PCR technique is a convenient and sensitive method in detecting HPV infection in human cervical carcinoma.It can be used in epidemic investigation and early findings of cervical carcinoma.