胚胎骨骨诱导活性的实验研究

An Experimental Study on Osteoinductive Property of Embryonic Bone

制备人胎骨全脱钙与部分脱钙骨基质明胶（简称ＢＭＧ），并分别做成颗粒（直径０．５～１ｃｍ）和粉末（直径＜２５０μｍ），同时制备少许未脱钙的胎骨颗粒，植入大鼠双侧后肢的股肌陷窝内，猪骨ＢＭＧ作为对照。通过组织学切片来比较各组植入物的诱导成骨活性。结果显示：（１）胎骨ＢＭＧ成骨过程早且量多，免疫排斥反应低；（２）未脱钙的胚胎骨无诱导成骨活性；（３）猪骨ＢＭＧ有一定的成骨能力，但时间较晚，量少，早期有明显的免疫反应；（４）全脱钙和部分脱钙胎骨的诱导成骨活性接近（Ｐ＞０．０５）；（５）粉末脱钙胎骨ＢＭＧ的诱导成骨活性降低；

Completely and partially decalcified human embryonic bone matrix gelatin (BMG) was prepared into particles (diameter 0.5～1cm)and powder (diameter< 250μm) Concomitantly, embryonic bone particles without being demineralised were prepared. These preparations were implanted into rats′ thigh muscles. Porcine BMG was used for control. Their osteoinductive property was evaluated by histology. The results were as follows:(1) Chondrogenesis was observed at the sites of embryonic BMG implants at early stage, with weakly immunological response and significant chondrogenesis.(2) Embryonic bone particles without demineralization have no osteoinductive property.(3) Porcine bone BMG has weak osteoinductive property, with apparent immunological response at early stage.(4) Completely and partially decalcified BMG have simlilar osteoinductive activity( P >0 05).(5) Bone inductive activity of BMG powder ne drops.(6) BMG can be sterilized by alcohol.