裸鼠皮下泡型棘球蚴病移植瘤模型的建立

Establishment of a subcutaneous xenograft tumor model of alveolar echinococcosis in nude mice

目的建立裸鼠皮下泡型棘球蚴病移植瘤模型,为进一步深入研究泡型棘球蚴病奠定基础。方法选取25只裸鼠进行20%泡型棘球蚴原头节混悬液0.1 ml颈背部皮下注射接种,观察记录病灶生长状况,术后6个月行台盼蓝染色观察原头节细胞活性。结果术后8 d左右可见皮下病灶结节;模型建立3个月后裸鼠存活率为92%(23/25),泡型棘球蚴感染率为100%(23/23);少量原头节死亡,台盼蓝染色蓝色,原头节细胞活力为98%。结论泡型棘球蚴能够成功移植于裸鼠皮下,可为进一步建立泡型棘球蚴病的动物模型提供参考。

Objective To establish a subcutaneous xenograft tumor model of alveolar echinococcosis in nude mice,and to lay a foundation for further research on alveolar echinococcosis. Methods A total of 25 nude mice were given subcutaneous injection of 0. 1 ml 20% Echinococcus multilocularis protoscolex suspension at the back of the neck. The growth of lesions was observed and recorded,and trypan blue staining was performed at 6 months after injection to observe the cell viability of protoscolices. Results Subcutaneous lesions were observed at about 8 days after injection. At 3 months after the establishment of the model,the survival rate of the nude mice was 92%（ 23/25）,and the infection rate of Echinococcus multilocularis was 100%（ 23/23）. A small number of protoscolices died and were blue on trypan blue staining,and the cell viability of protoscolices was 98%. Conclusion Echinococcus multilocularis can be successfully transplanted to the subcutaneous part of nude mice,which provides a reference for further studies on the establishment of animal models of alveolar echinococcosis.