摘要
目的建立裸鼠皮下泡型棘球蚴病移植瘤模型,为进一步深入研究泡型棘球蚴病奠定基础。方法选取25只裸鼠进行20%泡型棘球蚴原头节混悬液0.1 ml颈背部皮下注射接种,观察记录病灶生长状况,术后6个月行台盼蓝染色观察原头节细胞活性。结果术后8 d左右可见皮下病灶结节;模型建立3个月后裸鼠存活率为92%(23/25),泡型棘球蚴感染率为100%(23/23);少量原头节死亡,台盼蓝染色蓝色,原头节细胞活力为98%。结论泡型棘球蚴能够成功移植于裸鼠皮下,可为进一步建立泡型棘球蚴病的动物模型提供参考。
Objective To establish a subcutaneous xenograft tumor model of alveolar echinococcosis in nude mice,and to lay a foundation for further research on alveolar echinococcosis. Methods A total of 25 nude mice were given subcutaneous injection of 0. 1 ml 20% Echinococcus multilocularis protoscolex suspension at the back of the neck. The growth of lesions was observed and recorded,and trypan blue staining was performed at 6 months after injection to observe the cell viability of protoscolices. Results Subcutaneous lesions were observed at about 8 days after injection. At 3 months after the establishment of the model,the survival rate of the nude mice was 92%( 23/25),and the infection rate of Echinococcus multilocularis was 100%( 23/23). A small number of protoscolices died and were blue on trypan blue staining,and the cell viability of protoscolices was 98%. Conclusion Echinococcus multilocularis can be successfully transplanted to the subcutaneous part of nude mice,which provides a reference for further studies on the establishment of animal models of alveolar echinococcosis.
作者
周启锋
张灵强
廖梦娇
庞明泉
李梦轩
任利
樊海宁
ZHOU Qifeng, ZHANG Lingqiang, LIAO Mengjiao, et al.(Medical College of Qinghai University, Xining 810001, Chin)
出处
《临床肝胆病杂志》
CAS
北大核心
2018年第3期588-590,共3页
Journal of Clinical Hepatology
基金
青海大学附属医院中青年科研基金重点项目(ASRF-2016-ZD-02)
青海省科技计划项目(2014-ZJ-719)
关键词
棘球蚴病
肝
疾病模型
动物
echinococcosis, hepatic
disease models, animal