绿豆象触角转录组及嗅觉相关基因的分析

Analysis of the antennal transcriptome and olfaction-related genes of Callosobruchus chinensis ( Coleoptera: Bruchuidae)

【目的】建立绿豆象Callosobruchus chinensis触角转录组数据库,挖掘绿豆象的基因数据。【方法】采用高通量测序平台(Illumina Hiseq)对绿豆象成虫触角进行转录组测序、序列组装及生物信息学分析。【结果】共获得51.10 Gb的clean data(NCBI SRA数据库登录号:SRP119884)及83 535条unigenes,长度在1 kb以上的unigenes有15 075条,unigenes的N50为1 492 bp。使用BLAST软件将绿豆象触角Unigene序列与公共数据库比对,共注释到22 148条unigenes,其中NR数据库注释的unigenes最多,为18 744条,且与赤拟谷盗Tribolium castaneum的相似基因最多,达39.57%。通过GO数据库注释,将unigenes功能分为细胞组分、分子功能与生物学过程三大类54个分支,其中参与代谢进程以及结合与催化活性的unigenes比例较大。KEGG代谢途径分析表明,9 084条unigenes形成289条代谢通路,其中核糖体代谢通路所含unigene最多,为516条。进一步基因注释分析筛选到140个嗅觉相关基因,包括12个气味结合蛋白(odorant binding protein,OBP)基因,4个化学感受蛋白(chemosensory protein,CSP)基因,116个气味受体(odorant receptor,Or)基因,1个离子型受体(ionotropic receptor,IR)基因和7个感觉神经元膜蛋白(sensory neuron membrane protein,SNMP)基因,并用FPKM值对基因表达量进行评估。【结论】本研究获得了绿豆象触角转录组数据,为进一步研究绿豆象的基因功能分析及嗅觉感受机制奠定分子基础。

[ Aim] This study aims to establish the antennal transcriptome database of the azuki bean weevil, Callosobruchus chinensis, and to obtain the genetic data. [ Methods ] The antennal transcriptome of C. chinensis adults was sequenced using an Illumina HiSeq 4000 platform and subjected to bioinformatics analysis. [ Results] A total of 51.10 Gb clean data （NCBI SRA accession no. ： SRPl19884） were obtained, and the clean reads were assembled into 83 535 unigenes with an N50 length of 1 492 bp and 15 075 unigenes over 1 kb in length. A total of 22 148 unigenes in the antennal transcriptome of C. chinensis adults were annotated using BLASTX search against the public databases. Most unigenes （ 18 744 unigenes） were annotated to NR database, and the unigenes of C. chinensis had the highest similarity （39.57%） with those of Tribolium castaneum. All unigenes were divided into 54 branches of three categories （ cellular components, molecular function and biological processes） using Gene Ontology （GO） , and a lot of unigenes were annotated to be related to metabolic processes, binding activities and cellular processes. In KEGG database, 9 084 unigenes were assigned to 289 known metabolic pathways. Among these, most unigenes （516） are involved in metabolic pathways of ribosome. By further screening and identification, 140 olfaction-related genes of C. chinensis including 12 odorant binding protein （OBP） genes, 4 chemosensory protein （CSP） genes, 116 odorant receptor （OR） genes, 1 ionotropic receptor （IR） gene and 7 sensory neuron membrane protein （SNMP） genes were identified, and their expression levels were evaluated based on FPKM value. [ Conclusion ] This study obtained the antennal transcriptome data of C. chinensis. The results provide a foundation for the further study of gene functions and molecular mechanisms of olfactory reception of C. chinensis.