摘要
【目的】建立绿豆象Callosobruchus chinensis触角转录组数据库,挖掘绿豆象的基因数据。【方法】采用高通量测序平台(Illumina Hiseq)对绿豆象成虫触角进行转录组测序、序列组装及生物信息学分析。【结果】共获得51.10 Gb的clean data(NCBI SRA数据库登录号:SRP119884)及83 535条unigenes,长度在1 kb以上的unigenes有15 075条,unigenes的N50为1 492 bp。使用BLAST软件将绿豆象触角Unigene序列与公共数据库比对,共注释到22 148条unigenes,其中NR数据库注释的unigenes最多,为18 744条,且与赤拟谷盗Tribolium castaneum的相似基因最多,达39.57%。通过GO数据库注释,将unigenes功能分为细胞组分、分子功能与生物学过程三大类54个分支,其中参与代谢进程以及结合与催化活性的unigenes比例较大。KEGG代谢途径分析表明,9 084条unigenes形成289条代谢通路,其中核糖体代谢通路所含unigene最多,为516条。进一步基因注释分析筛选到140个嗅觉相关基因,包括12个气味结合蛋白(odorant binding protein,OBP)基因,4个化学感受蛋白(chemosensory protein,CSP)基因,116个气味受体(odorant receptor,Or)基因,1个离子型受体(ionotropic receptor,IR)基因和7个感觉神经元膜蛋白(sensory neuron membrane protein,SNMP)基因,并用FPKM值对基因表达量进行评估。【结论】本研究获得了绿豆象触角转录组数据,为进一步研究绿豆象的基因功能分析及嗅觉感受机制奠定分子基础。
[ Aim] This study aims to establish the antennal transcriptome database of the azuki bean weevil, Callosobruchus chinensis, and to obtain the genetic data. [ Methods ] The antennal transcriptome of C. chinensis adults was sequenced using an Illumina HiSeq 4000 platform and subjected to bioinformatics analysis. [ Results] A total of 51.10 Gb clean data (NCBI SRA accession no. : SRPl19884) were obtained, and the clean reads were assembled into 83 535 unigenes with an N50 length of 1 492 bp and 15 075 unigenes over 1 kb in length. A total of 22 148 unigenes in the antennal transcriptome of C. chinensis adults were annotated using BLASTX search against the public databases. Most unigenes ( 18 744 unigenes) were annotated to NR database, and the unigenes of C. chinensis had the highest similarity (39.57%) with those of Tribolium castaneum. All unigenes were divided into 54 branches of three categories ( cellular components, molecular function and biological processes) using Gene Ontology (GO) , and a lot of unigenes were annotated to be related to metabolic processes, binding activities and cellular processes. In KEGG database, 9 084 unigenes were assigned to 289 known metabolic pathways. Among these, most unigenes (516) are involved in metabolic pathways of ribosome. By further screening and identification, 140 olfaction-related genes of C. chinensis including 12 odorant binding protein (OBP) genes, 4 chemosensory protein (CSP) genes, 116 odorant receptor (OR) genes, 1 ionotropic receptor (IR) gene and 7 sensory neuron membrane protein (SNMP) genes were identified, and their expression levels were evaluated based on FPKM value. [ Conclusion ] This study obtained the antennal transcriptome data of C. chinensis. The results provide a foundation for the further study of gene functions and molecular mechanisms of olfactory reception of C. chinensis.
作者
郑海霞
张耀文
张仙红
杨美红
ZHENG Hai-Xia1, ZHANG Yao-Wen2, ZHANG Xian-Hong1 YANG Mei-Hong3(1. College of Agriculture, Shanxi Agricultural University, Taigu, Shanxi 030801, China; 2. Crop Research Institute of Shanxi Academy of Agricultural Sciences, Taiyuan 030000, China ; 3. Institute of Chemical Ecology, College of Arts and Sciences. Shanxi A~ricultural University. Taizu. Shanxi 030801. Chin)
出处
《昆虫学报》
CAS
CSCD
北大核心
2018年第2期168-177,共10页
Acta Entomologica Sinica
基金
现代农业产业技术体系专项(CARS-08-G10)
晋西北杂粮优质绿色关键技术研究与示范(201703D211002-8)
山西农业大学博士引进人才支持计划项目(2015ZZ11)
山西省自然科学基金项目(201601D011074)
关键词
绿豆象
触角转录组
高通量测序
基因注释
嗅觉相关基因
Callosobruchus chinensis
antennal transcriptome
HiSeq
gene annotation
olfaction-relatedgenes