响应面法优化天葵子总生物碱提取工艺及其体外抗氧化活性研究

Optimization on Total Alkaloids Extraction from Semiaquilegia Adoxoides using Response Surface Methodology and Evaluation of Its Antioxidant Activity in vitro

目的采用响应面法优化超声波辅助提取天葵子总生物碱的工艺,并探讨其体外抗氧化活性。方法利用Design Expert 8.0.6软件设计,以乙醇浓度、液料比、超声时间、超声功率为考察因素,进行4因素3水平的响应面研究。采用DPPH法、ABTS法和总还原能力测定法进行体外抗氧化研究。结果所得的最佳提取工艺条件为:乙醇浓度为86%,液料比为37∶1 mL·g^(-1),超声时间为32 min,超声功率为140 W。提取物有一定的还原力,清除DPPH自由基的IC_(50)值为0.631 mg·L^(-1),清除ABTS自由基的IC_(50)值为0.747 mg·L^(-1)。结论所优选的工艺条件简便可行,抗氧化活性大小与总生物碱的质量浓度呈明显的量效关系。

Objective The total alkaloid extraction of Semiaquilegia adoxoides was optimized by ultrasonic assisted extraction, and the antioxidant activity of total alkaloids was analyzed in vitro. Methods Using Design Expert 8.0.6,the response surface test of ethanol concentration,liquid to solid ratio,ultrasonic time and ultrasonic power were assessed. DPPH method,ABTS method and total reduction ability were used to investigate in vitro antioxidant activities. Results The optimum extraction conditions were as follows：ethanol concentration was 86 %,liquid to solid ratio was 37∶1 mL · g^（-1）;an ultrasonic time of 32 min,and an ultrasonic power of 140 W. The IC_（50） of DPPH free radicals was 0.631 mg · L^（-1）,the IC_（50） value of scavenging ABTS free radical was 0.747 mg · L^（-1）. Conclusion The optimized extraction conditions were stable and feasible, and the concentration of total alkaloids and the antioxidant activities showed an obvious dose-effect relationship.