2016年婴幼儿轮状病毒腹泻感染特征分析

Analysis of diarrhea and features of infections in infant infected with rotavirus in 2016

目的研究2016年本院婴幼儿轮状病毒腹泻感染特征,为疾病防控提供指导。方法收集2016年本院腹泻婴幼儿患者临床资料,采集患儿粪便样品,HRV PAGE阳性者为婴幼儿轮状病毒感染。采用RT-PCR技术对患儿感染轮状病毒进行基因分型。结果 313份婴幼儿腹泻患者粪便样品中,轮状病毒阳性230份,阳性率73.48%。男性患儿167例,感染131例,感染率78.44%;女性患儿146例,感染99例,感染率67.81%,性别间感染率差异有统计学意义(χ2=4.5214,P<0.05)。1~2月和10月~12月流行季节患儿224例,轮状病毒感染193例,感染率86.16%;3月~9月非流行季节患儿89例,感染37例,感染率41.57%,差异有统计学意义(χ2=64.9840,P<0.05)。0~岁患儿169例,感染149例,感染率64.50%;1~岁患儿93例,感染56例,感染率60.22%;2~岁患儿51例,感染25例,感染率49.02%,不同年龄患儿感染率差异有统计学意义(χ2=40.6439,P<0.05)。230份粪便样品中G基因型阳性198份,阳性率86.09%,其中G[3]型阳性147份,G[1]型阳性51份;P基因阳性178份,阳性率77.39%,其中P[8]型阳性133份,P[4]型阳性45份。结论 2016年本院轮状病毒腹泻男性患者居多,流行季节仍为发病高峰期,低年龄患儿感染率较高;轮状病毒基因型以G[3]P[8]为主。

Objective To study diarrhea and the features of infection in infants infected with rotavirus in this hospital in 2016 in order to guide the prevention and control of disease. Methods Clinical data on and fecal samples from pediatric patients with diarrhea seen in this hospital in 2016 were collected.Patients who tested positive for human rotavirus（HRV）according to PAGE were deemed to be pediatric patients infected with rotavirus.The genotype of rotavirus infecting pediatric patients was analyzed using RT-PCR. Results Of 313 fecal samples from pediatric patients with diarrhea,230 were infected with rotavirus at a rate of 73.48%.Of 167 male pediatric patients,131 were infected with HRV,for a rate of infection of 78.44%.Of 146 female pediatric patients,99 were infected with HRV,for a rate of infection of67.81%.The rate of infection differed significantly（χ2=4.5214,P〈0.05）by gender.Of 224 pediatric patients,193 were infected with rotavirus during stomach flu season in January-February and October-December,and their rate of infection was 86.16%.Of 89 pediatric patients,37 were infected with rotavirus outside of stomach flu season in March-September,and their rate of infection was 41.57%.The rate of infection differed significantly（χ2=64.9840,P〈0.05）by season.Of 169 pediatric patients under the age of 1,149 were infected with rotavirus,and their rate of infection was 64.50%.Of 93 pediatric patients up to 1 year of age,56 were infected with rotavirus,and their rate of infection was 60.22%.Of 51 pediatric patients up to 2 years of age,25 were infected with rotavirus,and their rate of infection was 49.02%.The rate of infection differed significantly（χ2=40.6439,P〈0.05）by age.The G genotype was detected in 198 of 230 fecal samples at a rate of 86.09%.The G[3]type was detected in 147 samples and the G [1]type was detected in 51.The P gene was detected in 178 samples at a rate of 77.39%.The P[8]type was detected in 133 samples and the P[4]type was detected in 45. Conclusion More male patients with diarrhea were infected with rotavirus in 2016.Incidence peaked during stomach flu season,and younger children had a high rate of infection.The genotype of rotavirus was mainly G[3]P[8].