人脐带间充质干细胞对耐亚胺培南铜绿假单胞菌生长的抑制作用

Inhibitory effect of human umbilical cord mesenchymal stem cells(hUCMSCs) against Pseudomonas aeruginosa

目的探讨人脐带间充质干细胞(h UCMSCs)是否抑制耐亚胺培南铜绿假单胞菌(IRPA)的生长。方法将IRPA菌液加入h UCMSCs细胞悬液中设为实验组,将IRPA菌液加入成纤维细胞细胞悬液中为对照组,共培育6 h后采用平板计数法对两组IRPA进行计数并比较;采用蛋白免疫印记技术和酶联免疫法检测两组共培育上清液中抗菌肽LL-37(Cathelicidin/LL-37)及人β防御素2(HBD-2)。结果实验组中的细菌集落数(CFU)低于对照组,差异具有统计学意义[(2.63±0.58)×106 CFU/ml vs.(3.69±0.91)×106 CFU/ml,t=19.93、P=0.031]。实验组共培育液中抗菌肽LL-37和HBD-2浓度高于对照组,差异具有统计学意义[抗菌肽LL-37:(7.99±0.45)ng/ml vs.(0.18±0.04)ng/ml,t=78.30、P=0.007;HBD-2:(249.38±14.19)pg/ml vs.(1.00±0.58)pg/ml,t=78.23、P=0.009]。结论人脐带间充质干细胞对耐亚胺培南铜绿假单胞菌生长起抑制作用,其可能通过分泌抗菌肽LL-37和HBD-2来实现。

Objective To investigate inhibitory effect of the human umbilical cord mesenchymal stem cells（hUCMSCs）on the growth of imipenem-resistant Pseudomonas aeruginosa（IRPA）.Methods IRPA added into hUCMSCs were collected as the experimental group; while the IRPA co-cultured with fibroblast were taken as the control group. After 6 hours of co-cultivation, colony forming units （CFUs） of IRPA were counted in each group using plate counting method. Then the levels of Cathelicidin/LL-37 and human β-defensin 2 （HBD-2） in cultured medium were detected by Western-blot and ELISA. Results The CFUs of IRPA in experimental group were less than those in control groups,with significant differences（2.63 ± 0.58）×106CFU/ml vs.（3.69 ± 0.91）× 106CFU/ml; t = 19.93, P 〈 0.05）. Cathelicidin/LL-37 and hBD2 in experimental group were higher than those of experimental group[Cathelicidin/LL-37：（7.99 ± 0.45）ng/ml vs.（0.18 ± 0.04）ng/ml;t=78.30,P=0.007;HBD-2：（249.38 ± 14.19）pg/ml vs.（1.00 ± 0.58）pg/ml;t=78.23,P=0.009].Conclusions hUCMSCs plays an inhibitory role on the growth of IRPA. The possible mechanism is that hUCMSCs could produce antimicrobial peptide：Cathelicidin/LL-37 and hBD2.