改良式大鼠同种异体肾移植纤维化加快模型的建立

Advanced establishment of an accelerated rat renal allograft fibrosis model

目的通过强化冷缺血再灌注损伤及改进手术方式建立大鼠同种异体肾移植纤维化加快模型,分析其效果。方法 SD大鼠为供体,Wistar大鼠为受体,摘取供肾后置于4℃肝素生理盐水中强化冷缺血1h,供体带肾下腔静脉与受体肾静脉用Cuff管进行吻合;供体带肾腹主动脉与受体腹主动脉进行连续吻合;供体带输尿管膀胱瓣与受体膀胱进行吻合。移植后8周取血查肾功,HE染色及Masson染色进行病理观察。结果实验组手术平均时间明显低于对照组(100±16)vs(124±15)min,移植成功率高于对照组(85%vs 70%);实验组血Scr水平明显高于对照组(165.98±12.54)vs(125±14.38)μmol/L(P<0.01);实验组血BUN水平也高于对照组,(59.62±8.98)vs(51.26±10.07)mg/dL,差异均有统计学意义(P<0.05);实验组肾实质慢性炎症反应明显,细胞外基质沉积多于对照组。结论通过强化供肾冷缺血及改进手术方式能有效建立肾移植纤维化加快模型,缩短手术时间,提高移植成功率。

Objective To establish an accelerated rat renal allograft fibrosis model via intensifying the cold ischemia reperfusion injury and modifying surgical methods. Methods SD rats were taken as donors and Wistar rats as recipients. The donor＇s kidney was put in 4℃ heparin saline for lh in order to intensity cold ischemia. The donor＇s kidney inferior vena cava and the recipient＇s kidney vein were anastomosed with Cuff tube. The donor＇s kidney ab- dominal aorta and the recipient＇s abdominal aorta were anastomosed continuously. The donor＇s ureter-bladder was anastomosed to the recipient bladder. At 8 weeks after transplantation,the Scr and BUN were detected using blood, and the pathological changes were absolved by HE and Masson staining. Results The average operation time of the experimental group was significantly lower than that of the control group （100 ± 16 vs 124± 15 min）, and the suc- cess rate of transplantation was higher than that of the control group （850//oo vs 70%）. The Scr in experimental group was significantly higher than that in the control group （165.98＋12.54 vs 125±14.38μmol/L,P〈0.01） ,so as the blood BUN （59.62±8.98 vs 51.26±10.07 mg/dL,P〈0. 05）. There＇s obvious chronic inflammatory exist in experimental group,and much more ECM deposited in renal interstitum than that in the control group. Conclusion Accelerated rat renal allograft fibrosis model can be effectively established via intensifying the cold ischemia reperfusion injury and modifying surgical methods,and the operation time is shortened and the graft success rate is improved.