国产巴西人参醋酸乙酯部位化学成分研究

Chemical constituents from ethyl acetate extracts of domestic Pfaffia glomerata

目的研究国产巴西人参Pfaffia glomerata醋酸乙酯部位的化学成分。方法利用硅胶、反相树脂、Sephadex LH-20凝胶等柱色谱分离纯化,通过理化性质和波谱数据鉴定化合物结构。结果从国产巴西人参醋酸乙酯部位分离得到20个化合物,分别鉴定为正十一醇(1)、油酸(2)、β-谷甾醇(3)、2-羟基-4,6-二甲氧基苯乙酮(4)、齐墩果酸(5)、pfaffianol A(6)、对苯二酚(7)、香草酸(8)、iresinone(9)、3,4-二羟基肉桂酸乙酯(10)、齐墩果酸-28-O-β-D-吡喃葡萄糖酯苷(11)、尿囊素(12)、筋骨草甾酮C(13)、β-蜕皮甾酮(14)、iresinoside(15)、腺嘌呤(16)、齐墩果酸-3-O-β-D-葡萄糖醛酸苷(17)、ficusoside B(18)、pfaffiaglycoside B(19)、β-D-吡喃葡萄糖基-3-(O-β-D-吡喃葡萄糖氧基)-齐墩果酸酯(20)。结论其中化合物7~10、15、17、18、20为首次从该植物中分离得到。

Objective To study the chemical constituents of ethyl acetate extract from domestic Pfaffia glomerata. Methods The compounds were isolated and purified by silica gel, reversed-phase, Sephadex LH-20 column chromatography, and their structures were elucidated by spectroscopic analysis as well as chemical methods. Results Twenty compounds were identified as 1-undecanol（1）, oleic acid（2）, β-sitosterol（3）, 2-hydroxy-4,6-dimethoxyacetophenone（4）, oleanolic acid（5）, pfaffianol A（6）, benzene-1,4-diol（7）, vanillic acid（8）, iresinone（9）, ethylcaffeate（10）, oleanoicaeid-28-O-β-D-glucopyranosyl ester（11）, allantoin（12）, ajugasterone-C（13）, β-ecdysterone（14）, iresinoside（15）, adenine（16）, oleanolic acid 3-O-β-D-glucuronopyranoside（17）, ficusoside B（18）, pfaffiaglycoside B（19）, and β-D-Glucopyranosyl-3-（O-β-D-glucopyranosyloxy）-oleanolate（20）. Conclusion Compounds 7—10, 15, 17, 18, and 20 are isolated from this plant for the first time.